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验证数据展示

  • WB analysis of A431 using 19806-1-AP

    WB analysis of A431 using 19806-1-AP

    WB result of TRIM35 antibody (19806-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM35 transfected A431 cells.

  • WB analysis using 19806-1-AP

    WB analysis using 19806-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19806-1-AP (TRIM35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 19806-1-AP

    IHC staining of human liver using 19806-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19806-1-AP (TRIM35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 19806-1-AP

    IHC staining of human liver using 19806-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19806-1-AP (TRIM35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 19806-1-AP

    IHC staining of mouse liver using 19806-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19806-1-AP (TRIM35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 19806-1-AP

    IF Staining of U2OS using 19806-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TRIM35 antibody (19806-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

TRIM35 Polyclonal antibody

TRIM35 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19806-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

E3 ubiquitin-protein ligase TRIM35, EC:2.3.2.27, HLS5, KIAA1098, tripartite motif containing 35

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inH9C2 cells, A431 cells, HEK-293 cells
Positive IHC detected inhuman liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19806-1-AP targets TRIM35 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13584

Product name: Recombinant human TRIM35 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-206 aa of BC018337

Sequence: MERSPDVSPGPSRSFKEELLCAVCYDPFRDAVTLRCGHNFCRGCVSRCWEVQVSPTCPVCKDRASPADLRTNHTLNNLVEKLLREEAEGARWTSYRFSRVCRLHRGQLSLFCLEDKELLCCSCQADPRHQGHRVQPVKDTAHDFRVRSLIAEERRNFLPTHQWIVTKTRLQTSSPNLQSRRQGQVQEHGACTAGEGQGLLGHAALL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 35
别名 E3 ubiquitin-protein ligase TRIM35, EC:2.3.2.27, HLS5, KIAA1098, tripartite motif containing 35
计算分子量 57 kDa
观测分子量45~50 kDa
GenBank蛋白编号BC018337
基因名称 TRIM35
Gene ID (NCBI) 23087
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPQ4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tripartite motif 35 (TRIM35), also called hemopoietic lineage switch (Hls5), is a member of the RING finger, B box, coiled-coil (RBCC), or TRIM family E3 ligases expressed in a wide variety of hemopoietic cell types, including fetal liver progenitors. TRIM35 is reported to be a tumor suppressor gene and is expressed at low levels in several types of cancer (PMID: 34124276, 35081724). Western detected a band at 45-50 kDa possibly due to the high proline content.

实验方案

Product Specific Protocols
IF protocol for TRIM35 antibody 19806-1-APDownload protocol
IHC protocol for TRIM35 antibody 19806-1-APDownload protocol
WB protocol for TRIM35 antibody 19806-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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