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验证数据展示

  • WB analysis of K-562 using 83316-2-RR (same clone as 83316-2-PBS)

    WB analysis of K-562 using 83316-2-RR (same clone as 83316-2-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 83316-2-RR (TRIM34 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83316-2-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 83316-2-RR (same clone as 83316-2-PBS)

    WB analysis of human placenta using 83316-2-RR (same clone as 83316-2-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 83316-2-RR (TRIM34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83316-2-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 83316-2-RR (same clone as 83316-2-PBS)

    IF Staining of HEK-293 using 83316-2-RR (same clone as 83316-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TRIM34 antibody (83316-2-RR, Clone: 240180E2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83316-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00340-3

    Cytometric bead array standard curve of MP00340-3

    Cytometric bead array standard curve of MP00340-3, TRIM34 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83316-2-PBS. Detection antibody: 83316-3-PBS. Standard: Ag34444. Range: 1.25-40 ng/mL.

  • Affinity and Kinetic Characterization of 83316-2-RR

    Affinity and Kinetic Characterization of 83316-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83316-2-RR against Human TRIM34 were performed. The affinity constant is 0.941 nM.

TRIM34 Recombinant antibody, PBS Only (Capture)

TRIM34 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83316-2-PBS

产品说明书

CloneNo. 240180E2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

240180E2, IFP1, RNF21, tripartite motif containing 34

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83316-2-PBS targets TRIM34 as part of a matched antibody pair:

MP00340-3: 83316-2-PBS capture and 83316-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 TRIM34 fusion protein Ag34444 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 34
别名 240180E2, IFP1, RNF21, tripartite motif containing 34
计算分子量57 kDa
观测分子量31 kDa, 57 kDa
GenBank蛋白编号NM_001003827
基因名称 TRIM34
Gene ID (NCBI) 53840
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BYJ4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tripartite motif 34 (TRIM34) is a member of TRIM family that can be highly induced by type I Interferon. TRIM34 has a stimulative role in cell apoptosis and a suppressive role in inflammation. TRIM34 serves a tumor suppressor role in the occurrence and development of colon cancer by enhancing cell apoptosis. TRIM34 possesses at least three kinds of isoforms: the long form at 97 kDa, medium form at 57 kDa, the short form 31 kDa (PMID: 11013086, 31956709).