验证数据展示

WB analysis using 10326-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IF Staining of mouse embryo using 10326-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using TRIM32 antibody (10326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
WB analysis of mouse colon using 10326-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10326-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IF Staining of hTERT-RPE1 cells using 10326-1-AP
IF result (cytoplasm stain) of anti-BBS11 (10326-1-AP; 1:50) with hTERT-RPE1 cell by Dr. Moshe Kim.
IP experiment of U-251 using 10326-1-AP
IP result of anti-TRIM32 (IP:10326-1-AP, 4ug; Detection:10326-1-AP 1:2000) with U-251 cells lysate 1200 ug.
IHC staining of human colon using 10326-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10326-1-AP (TRIM32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TRIM32 antibody included
IHC staining of human colon using 10326-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10326-1-AP (TRIM32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TRIM32 antibody included
IF Staining of hTERT-RPE1 using 10326-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using TRIM32 antibody (10326-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
FC experiment of A431 using 10326-1-AP
1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TRIM32 (10326-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

TRIM32 Polyclonal antibody

TRIM32 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 10326-1-AP

产品说明书

引用文献(19)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (1)

别名

72 kDa Tat-interacting protein, E3 ubiquitin-protein ligase TRIM32, EC:2.3.2.27, HT2A, RING-type E3 ubiquitin transferase TRIM32

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (19)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A549 cells, mouse colon tissue, NIH/3T3 cells, HeLa cells
Positive IP detected in	U-251 cells
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse embryo tissue
Positive IF/ICC detected in	hTERT-RPE1 cells
Positive FC (Intra) detected in	A431 cells

Planning an IHC experiment? We recommend our IHCeasy TRIM32 Ready-To-Use IHC Kit. TRIM32 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:100-1:400
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 11 publications below
WB	See 15 publications below
IHC	See 4 publications below
IF	See 5 publications below
IP	See 1 publications below

产品信息

10326-1-AP targets TRIM32 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag0401 Product name: Recombinant human TRIM32 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 111-430 aa of BC003154 Sequence: FCRSCGLVLCEPCREADHQPPGHCTLPVKEAAEERRRDFGEKLTRLRELMGELQRRKAALEGVSKDLQARYKAVLQEYGHEERRVQDELARSRKFFTGSLAEVEKSNSQVVEEQSYLLNIAEVQAVSRCDYFLAKIKQADVALLEETADEEEPELTASLPRELTLQDVELLKVGHVGPLQIGQAVKKPRTVNVEDSWAMEATASAASTSVTFREMDMSPEEVVASPRASPAKQRGPEAASNIQQCLFLKKMGAKGSTPGMFNLPVSLYVTSQGEVLVADRGNYRIQVFTRKGFLKEIRRSPSGIDSFVLSFLGADLPNLT 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	tripartite motif-containing 32
别名	72 kDa Tat-interacting protein, E3 ubiquitin-protein ligase TRIM32, EC:2.3.2.27, HT2A, RING-type E3 ubiquitin transferase TRIM32
计算分子量	72 kDa
观测分子量	70-72 kDa
GenBank蛋白编号	BC003154
基因名称	TRIM32
Gene ID (NCBI)	22954
RRID	AB_2208005
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q13049
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390). Western blot analysis detected TRIM32 at an apparent molecular mass of 70-72 kDa.

实验方案

Product Specific Protocols
FC protocol for TRIM32 antibody 10326-1-AP	Download protocol
IF protocol for TRIM32 antibody 10326-1-AP	Download protocol
IHC protocol for TRIM32 antibody 10326-1-AP	Download protocol
IP protocol for TRIM32 antibody 10326-1-AP	Download protocol
WB protocol for TRIM32 antibody 10326-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,IHC	Cell Death Differ TRIM32 promotes anoikis resistance and metastasis in NSCLC by degrading CHEK2 to enhance IL-6 secretion Authors - Jia Xu KO Validated View Article
human	WB	Sci Adv An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis. Authors - Molly M Wilson View Article
mouse,human	IHC	J Am Soc Nephrol Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis. Authors - Sybille Koehler View Article
mouse	WB	Hum Mol Genet Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component. Authors - Kudryashova Elena E KO Validated View Article
human	WB	PLoS Pathog TRIM32 inhibits Venezuelan equine encephalitis virus infection by targeting a late step in viral entry Authors - Yifan Xie KD Validated View Article
rat,human	WB,IP,IF	FASEB J Ubiquitin ligase TRIM32 promotes dendrite arborization by mediating degradation of the epigenetic factor CDYL. Authors - Lei Liu KD Validated View Article

验证数据展示

WB analysis using 10326-1-AP

IF Staining of mouse embryo using 10326-1-AP

WB analysis of mouse colon using 10326-1-AP

WB analysis of NIH/3T3 using 10326-1-AP

IF Staining of hTERT-RPE1 cells using 10326-1-AP

IP experiment of U-251 using 10326-1-AP

IHC staining of human colon using 10326-1-AP

IHC staining of human colon using 10326-1-AP

IF Staining of hTERT-RPE1 using 10326-1-AP

FC experiment of A431 using 10326-1-AP

TRIM32 Polyclonal antibody

TRIM32 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

TRIM32 promotes anoikis resistance and metastasis in NSCLC by degrading CHEK2 to enhance IL-6 secretion

An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.

Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis.

Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component.

TRIM32 inhibits Venezuelan equine encephalitis virus infection by targeting a late step in viral entry

Ubiquitin ligase TRIM32 promotes dendrite arborization by mediating degradation of the epigenetic factor CDYL.

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