×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 10326-1-AP

    WB analysis using 10326-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse embryo using 10326-1-AP

    IF Staining of mouse embryo using 10326-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using TRIM32 antibody (10326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of mouse colon using 10326-1-AP

    WB analysis of mouse colon using 10326-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 10326-1-AP

    WB analysis of NIH/3T3 using 10326-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 cells using 10326-1-AP

    IF Staining of hTERT-RPE1 cells using 10326-1-AP

    IF result (cytoplasm stain) of anti-BBS11 (10326-1-AP; 1:50) with hTERT-RPE1 cell by Dr. Moshe Kim.

  • IP experiment of U-251 using 10326-1-AP

    IP experiment of U-251 using 10326-1-AP

    IP result of anti-TRIM32 (IP:10326-1-AP, 4ug; Detection:10326-1-AP 1:2000) with U-251 cells lysate 1200 ug.

  • IHC staining of human colon using 10326-1-AP

    IHC staining of human colon using 10326-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10326-1-AP (TRIM32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRIM32 antibody included
  • IHC staining of human colon using 10326-1-AP

    IHC staining of human colon using 10326-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10326-1-AP (TRIM32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRIM32 antibody included
  • IF Staining of hTERT-RPE1 using 10326-1-AP

    IF Staining of hTERT-RPE1 using 10326-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using TRIM32 antibody (10326-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • FC experiment of A431 using 10326-1-AP

    FC experiment of A431 using 10326-1-AP

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TRIM32 (10326-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO Validated

TRIM32 Polyclonal antibody

TRIM32 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 10326-1-AP

产品说明书

引用文献(22)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (1)

别名

72 kDa Tat-interacting protein, E3 ubiquitin-protein ligase TRIM32, EC:2.3.2.27, HT2A, RING-type E3 ubiquitin transferase TRIM32

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, mouse colon tissue, NIH/3T3 cells, HeLa cells
Positive IP detected inU-251 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse embryo tissue
Positive IF/ICC detected inhTERT-RPE1 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy TRIM32 Ready-To-Use IHC Kit. TRIM32 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:100-1:400
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10326-1-AP targets TRIM32 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag0401

Product name: Recombinant human TRIM32 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 111-430 aa of BC003154

Sequence: FCRSCGLVLCEPCREADHQPPGHCTLPVKEAAEERRRDFGEKLTRLRELMGELQRRKAALEGVSKDLQARYKAVLQEYGHEERRVQDELARSRKFFTGSLAEVEKSNSQVVEEQSYLLNIAEVQAVSRCDYFLAKIKQADVALLEETADEEEPELTASLPRELTLQDVELLKVGHVGPLQIGQAVKKPRTVNVEDSWAMEATASAASTSVTFREMDMSPEEVVASPRASPAKQRGPEAASNIQQCLFLKKMGAKGSTPGMFNLPVSLYVTSQGEVLVADRGNYRIQVFTRKGFLKEIRRSPSGIDSFVLSFLGADLPNLT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 32
别名 72 kDa Tat-interacting protein, E3 ubiquitin-protein ligase TRIM32, EC:2.3.2.27, HT2A, RING-type E3 ubiquitin transferase TRIM32
计算分子量 72 kDa
观测分子量70-72 kDa
GenBank蛋白编号BC003154
基因名称 TRIM32
Gene ID (NCBI) 22954
RRIDAB_2208005
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13049
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390). Western blot analysis detected TRIM32 at an apparent molecular mass of 70-72 kDa.

实验方案

Product Specific Protocols
FC protocol for TRIM32 antibody 10326-1-APDownload protocol
IF protocol for TRIM32 antibody 10326-1-APDownload protocol
IHC protocol for TRIM32 antibody 10326-1-APDownload protocol
IP protocol for TRIM32 antibody 10326-1-APDownload protocol
WB protocol for TRIM32 antibody 10326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nat Commun

Small intestinal γδ T17 cells promote SAE through STING/C1q-induced microglial synaptic pruning in male mice

Authors - Yuming Wu
View Article
humanWB,IF

Mol Cell

Opposing regulation of the STING pathway in hepatic stellate cells by NBR1 and p62 determines the progression of hepatocellular carcinoma

Authors - Sadaaki Nishimura
View Article
humanWB

Sci Adv

An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.

Authors - Molly M Wilson
View Article
humanWB,IHC

Cell Death Differ

TRIM32 promotes anoikis resistance and metastasis in NSCLC by degrading CHEK2 to enhance IL-6 secretion

Authors - Jia Xu
  • KO Validated
View Article
mouseWB,IF

Int J Biol Sci

TRIM32 promotes neuronal ferroptosis by enhancing K63-linked ubiquitination and subsequent p62-selective autophagic degradation of GPX4

Authors - Xin Zhou
  • KD Validated
View Article
mouse,humanIHC

J Am Soc Nephrol

Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis.

Authors - Sybille Koehler
View Article
||
New chat

Able™

正在加载，请稍候...