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验证数据展示

  • WB analysis using 28392-1-AP (same clone as 28392-1-PBS)

    WB analysis using 28392-1-AP (same clone as 28392-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28392-1-AP (TRIM3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28392-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 28392-1-AP (same clone as 28392-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 28392-1-AP (same clone as 28392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 28392-1-AP (TRIM3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28392-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 28392-1-AP (same clone as 28392-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 28392-1-AP (same clone as 28392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 28392-1-AP (TRIM3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28392-1-PBS in a different storage buffer formulation.

TRIM3 Polyclonal antibody, PBS Only

TRIM3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28392-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

RNF22, RING finger protein 97, RING finger protein 22, HAC1, FLJ16135

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28392-1-PBS targets TRIM3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28823

Product name: Recombinant human TRIM3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 187-324 aa of NM_001248006

Sequence: SQQLQERKAEALAQISAAFEDLEQALQQRKQALVSDLETICGAKQKVLQSQLDTLRQGQEHIGSSCSFAEQALRLGSAPEVLLVRKHMRERLAALAAQAFPERPHENAQLELVLEVDGLRRSVLNLGALLTTSATAHE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 3
别名 RNF22, RING finger protein 97, RING finger protein 22, HAC1, FLJ16135
计算分子量 81 kDa
观测分子量 75 kDa, 80-85 kDa
GenBank蛋白编号NM_001248006
基因名称 TRIM3
Gene ID (NCBI) 10612
RRIDAB_2881131
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75382
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRIM3, a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM family of genes is largely studied because of their roles in development, differentiation and host cell antiviral defenses. TRIM3 is a mammalian tumor suppressor whose loss can increase the incidence and promote the development of GBM(PMID: 23318451). TRIM3 has four isoforms with the MW of 67-80 kDa and can be detected as 75, 80, 82,85 kDa after posttranslational modification(PMID: 24947043/PMID: 24086586 ).

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