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  • WB analysis of HeLa using 15202-1-AP

    WB analysis of HeLa using 15202-1-AP

    WB result of KAP1 antibody (15202-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KAP1 transfected HeLa cells.

  • IP experiment of HeLa using 15202-1-AP

    IP experiment of HeLa using 15202-1-AP

    IP result of anti-KAP1 (IP:15202-1-AP, 4ug; Detection:15202-1-AP 1:1000) with HeLa cells lysate 1200ug.

  • WB analysis using 15202-1-AP

    WB analysis using 15202-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15202-1-AP (KAP1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 15202-1-AP

    IHC staining of human colon using 15202-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15202-1-AP (KAP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAP1 antibody included
  • IHC staining of human lung cancer using 15202-1-AP

    IHC staining of human lung cancer using 15202-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15202-1-AP (KAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAP1 antibody included
  • IHC staining of human lung cancer using 15202-1-AP

    IHC staining of human lung cancer using 15202-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15202-1-AP (KAP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAP1 antibody included
  • IF Staining of HeLa using 15202-1-AP

    IF Staining of HeLa using 15202-1-AP

    Immunofluorescent analysis of Hela cells, using TRIM28 antibody 15202-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IF Staining of HeLa using 15202-1-AP

    IF Staining of HeLa using 15202-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KAP1 antibody (15202-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HeLa using 15202-1-AP

    FC experiment of HeLa using 15202-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KAP1 (15202-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Featured Product
  • KD/KO Validated

KAP1 Polyclonal antibody

KAP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 15202-1-AP

产品说明书

引用文献(61)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

别名

TRIM28, E3 SUMO-protein ligase TRIM28, EC:2.3.2.27, KAP 1, KAP-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse testis tissue, HepG2 cells, L02 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KAP1 Ready-To-Use IHC Kit. KAP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15202-1-AP targets KAP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, chIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, pig, monkey
免疫原

CatNo: Ag7350

Product name: Recombinant human KAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 484-835 aa of BC004978

Sequence: KVPRVSLERLDLDLTADSQPPVFKVFPGSTTEDYNLIVIERGAAAAATGQPGTAPAGTPGAPPLAGMAIVKEEETEAAIGAPPTATEGPETKPVLMALAEGPGAEGPRLASPSGSTSSGLEVVAPEGTSAPGGGPGTLDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPANQRKCERVLLALFCHEPCRPLHQLATDSTFSLDQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPMSLPGAGLSSQELSGGPGDGP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 28
别名 TRIM28, E3 SUMO-protein ligase TRIM28, EC:2.3.2.27, KAP 1, KAP-1
计算分子量 89 kDa
观测分子量 100 kDa
GenBank蛋白编号BC004978
基因名称 KAP1
Gene ID (NCBI) 10155
RRIDAB_2209890
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13263
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )

实验方案

Product Specific Protocols
IF protocol for KAP1 antibody 15202-1-APDownload protocol
IHC protocol for KAP1 antibody 15202-1-APDownload protocol
IP protocol for KAP1 antibody 15202-1-APDownload protocol
WB protocol for KAP1 antibody 15202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cancer

ZDHHC20 mediated S-palmitoylation of fatty acid synthase (FASN) promotes hepatocarcinogenesis

Authors - Yaqi Mo
View Article
humanWB

Cancer Discov

Transcription Elongation Machinery Is a Druggable Dependency and Potentiates Immunotherapy in Glioblastoma Stem Cells

Authors - Zhixin Qiu
View Article
humanWB,CoIP

Gut

Targeting NEK9 synergises with immunotherapy in hepatocellular carcinoma by remodelling the immunosuppressive microenvironment

Authors - Guofang Lu
View Article
humanWB,IHC

Sci Bull (Beijing)

TRIM28 protects CARM1 from proteasome-mediated degradation to prevent colorectal cancer metastasis

Authors - Jinyuan Cui
View Article
humanWB

Nat Commun

RNAPII-dependent ATM signaling at collisions with replication forks

Authors - Elias Einig
View Article
humanWB

Nat Commun

URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers

Authors - Zhiwen Ding
View Article
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