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验证数据展示

  • IF Staining of HEK-293T using 67314-1-Ig (same clone as 67314-1-PBS)

    IF Staining of HEK-293T using 67314-1-Ig (same clone as 67314-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293T cells using TRIM25 antibody (67314-1-Ig, Clone: 1H6G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

  • WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

    WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

  • WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

    WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67314-1-Ig (same clone as 67314-1-PBS)

    IHC staining of human breast cancer using 67314-1-Ig (same clone as 67314-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRIM25 antibody included
  • IHC staining of human breast cancer using 67314-1-Ig (same clone as 67314-1-PBS)

    IHC staining of human breast cancer using 67314-1-Ig (same clone as 67314-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRIM25 antibody included
  • Featured Product
  • KD/KO验证

TRIM25 Monoclonal antibody, PBS Only

TRIM25 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67314-1-PBS

产品说明书

CloneNo. 1H6G1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

1H6G1, EC:2.3.2.27, EC:6.3.2.n3, EFP, Estrogen-responsive finger protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TRIM25 Ready-To-Use IHC Kit. TRIM25 primary antibody included.

产品信息

67314-1-PBS targets TRIM25 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29325

Product name: Recombinant human TRIM25 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 298-630 aa of BC016924

Sequence: SLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQELTPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALPSKLPTFGAPEQLVDLKQAGLEAAAKATSSHPNSTSLKAKVLETFLAKSRPELLEYYIKVILDYNTAHNKVALSECYTVASVAEMPQNYRPHPQRFTYCSQVLGLHCYKKGIHYWEVELQKNNFCGVGICYGSMNRQGPESRLGRNSASWCVEWFNTKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVADKVHLMYKFRVDFTEALYPAFWVFSAGATLSICSPK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tripartite motif-containing 25
别名 1H6G1, EC:2.3.2.27, EC:6.3.2.n3, EFP, Estrogen-responsive finger protein
计算分子量 630 aa, 71 kDa
观测分子量 68-71 kDa
GenBank蛋白编号BC016924
基因名称 TRIM25
Gene ID (NCBI) 7706
RRIDAB_2882574
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14258
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity (PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection (PMID: 17392790).

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