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验证数据展示

  • WB analysis using 66324-1-Ig (same clone as 66324-1-PBS)

    WB analysis using 66324-1-Ig (same clone as 66324-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66324-1-Ig (same clone as 66324-1-PBS)

    IHC staining of human breast cancer using 66324-1-Ig (same clone as 66324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRIM24 antibody included
  • IHC staining of human lung cancer using 66324-1-Ig (same clone as 66324-1-PBS)

    IHC staining of human lung cancer using 66324-1-Ig (same clone as 66324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRIM24 antibody included
  • IF Staining of HepG2 using 66324-1-Ig (same clone as 66324-1-PBS)

    IF Staining of HepG2 using 66324-1-Ig (same clone as 66324-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM24 antibody (66324-1-Ig, Clone: 1D6D9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

TRIM24 Monoclonal antibody, PBS Only

TRIM24 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66324-1-PBS

产品说明书

CloneNo. 1D6D9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

1D6D9, E3 ubiquitin-protein ligase TRIM24, EC:2.3.2.27, RING-type E3 ubiquitin transferase TIF1-alpha, TIF1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TRIM24 Ready-To-Use IHC Kit. TRIM24 primary antibody included.

产品信息

66324-1-PBS targets TRIM24 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5417

Product name: Recombinant human TRIM24 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 701-1050 aa of BC028689

Sequence: SKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIVKQESDEESRPQNANYPRSILTSLLLNSSQSSTSEETVLRSDAPDSTGDQPGLHQDNSSNGKSEWLDPSQKSPLHVGETRKEDDPNEDWCAVCQNGGELLCCEKCPKVFHLSCHVPTLTNFPSGEWICTFCRDLSKPEVEYDCDAPSHNSEKKKTEGLVKLTPIDKRKCERLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYSMYSKPEDFVADFRLIFQNCAEFNEPDSEVANAGIKLENYFEELLKNLYPEKRFPKPEFRNESEDNKFSDDSDDDFVQPRKKRLKSIEERQLLK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tripartite motif-containing 24
别名 1D6D9, E3 ubiquitin-protein ligase TRIM24, EC:2.3.2.27, RING-type E3 ubiquitin transferase TIF1-alpha, TIF1
计算分子量 117 kDa
观测分子量 116 kDa
GenBank蛋白编号BC028689
基因名称 TRIM24
Gene ID (NCBI) 8805
RRIDAB_2881705
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO15164
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) which is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.

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