验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HEK-293 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
12607-1-AP targets TRIM23 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag3302 Product name: Recombinant human TRIM23 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 275-574 aa of BC022510 Sequence: EHVPGTAENARSCIRAYFYDLHETLCRQEEMALSVVDAHVREKLIWLRQQQEDMTILLSEVSAACLHCEKTLQQDDCRVVLAKQEITRLLETLQKQQQQFTEVADHIQLDASIPVTFTKDNRVHIGPKMEIRVVTLGLDGAGKTTILFKLKQDEFMQPIPTIGFNVETVEYKNLKFTIWDVGGKHKLRPLWKHYYLNTQAVVFVVDSSHRDRISEAHSELAKLLTEKELRDALLLIFANKQDVAGALSVEEITELLSLHKLCCGRSWYIQGCDARSGMGLYEGLDWLSRQLVAAGVLDVA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tripartite motif-containing 23 |
| 别名 | ARFD1, ARD1, RNF46, GTP binding protein ARD 1, EC:2.3.2.27 |
| 计算分子量 | 574 aa, 64 kDa |
| 观测分子量 | 64 kDa |
| GenBank蛋白编号 | BC022510 |
| 基因名称 | TRIM23 |
| Gene ID (NCBI) | 373 |
| RRID | AB_2256643 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P36406 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIM23 antibody 12607-1-AP | Download protocol |
| WB protocol for TRIM23 antibody 12607-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J Energy stress promotes P-bodies formation via lysine-63-linked polyubiquitination of HAX1 | ||
Int J Biol Macromol Engineering the orthogonal ubiquitin E1-E2 pairs for identification of K27 chain linkage substrates |
