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验证数据展示

  • WB analysis using 83530-3-RR (same clone as 83530-3-PBS)

    WB analysis using 83530-3-RR (same clone as 83530-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83530-3-RR (TRIM21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83530-3-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 83530-3-RR (same clone as 83530-3-PBS)

    IHC staining of human colon cancer using 83530-3-RR (same clone as 83530-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83530-3-RR (TRIM21 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83530-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRIM21 antibody included
  • IF Staining of A549 using 83530-3-RR (same clone as 83530-3-PBS)

    IF Staining of A549 using 83530-3-RR (same clone as 83530-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TRIM21 antibody (83530-3-RR, Clone: 240491B2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83530-3-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83530-3-RR (same clone as 83530-3-PBS)

    FC experiment of HeLa using 83530-3-RR (same clone as 83530-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRIM21 Recombinant antibody (83530-3-RR, Clone:240491B2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83530-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83530-3-RR

    Affinity and Kinetic Characterization of 83530-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83530-3-RR against Human TRIM21 were performed. The affinity constant is 11 nM.

TRIM21 Recombinant monoclonal antibody, PBS Only

TRIM21 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83530-3-PBS

产品说明书

CloneNo. 240491B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

240491B2, 52 kDa ribonucleoprotein autoantigen Ro/SS-A, E3 ubiquitin-protein ligase TRIM21, EC:2.3.2.27, RING finger protein 81

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TRIM21 Ready-To-Use IHC Kit. TRIM21 primary antibody included.

产品信息

83530-3-PBS targets TRIM21 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag28377

Product name: Recombinant human TRIM21 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 176-475 aa of BC010861

Sequence: SRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGAQHFHSGKHYWEVDVTGKEAWDLGVCRDSVRRKGHFLLSSKSGFWTIWLWNKQKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSFYNITDHGSLIYSFSECAFTGPLRPFFSPGFNDGGKNTAPLTLCPLNIGSQGSTDY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 21
别名 240491B2, 52 kDa ribonucleoprotein autoantigen Ro/SS-A, E3 ubiquitin-protein ligase TRIM21, EC:2.3.2.27, RING finger protein 81
计算分子量 475 aa, 54 kDa
观测分子量52 kDa
GenBank蛋白编号BC010861
基因名称 TRIM21
Gene ID (NCBI) 6737
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP19474
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM21 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules. TRIM21 exists various isoforms and range molecular weight of isoforms is about 42-45kDa and 49-54 kDa.

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