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  • Featured Product
  • KD/KO Validated

TRIM21 Polyclonal antibody

TRIM21 Polyclonal Antibody for WB, IP, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IP, IF/ICC, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 12108-1-AP

Print datasheet

Synonyms

52 kDa Ro protein, RING finger protein 81, RNF81, Ro(SS A), RO52, SS A, SSA, SSA1, TRIM21, tripartite motif containing 21

验证数据展示

  • WB analysis using 12108-1-AP

    WB analysis using 12108-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 12108-1-AP

    IHC staining of human pancreas cancer using 12108-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12108-1-AP (TRIM21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRIM21 antibody included
  • IP experiment of A549 using 12108-1-AP

    IP experiment of A549 using 12108-1-AP

    IP result of anti-TRIM21 (IP:12108-1-AP, 4ug; Detection:12108-1-AP 1:500) with A549 cells lysate 920ug.

  • WB analysis using 12108-1-AP

    WB analysis using 12108-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 12108-1-AP

    WB analysis using 12108-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 12108-1-AP

    WB analysis of HeLa using 12108-1-AP

    WB result of TRIM21 antibody (12108-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM21 transfected HeLa cells.

  • IF Staining of A549 using 12108-1-AP

    IF Staining of A549 using 12108-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRIM21 antibody (12108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A549 using 12108-1-AP

    IF Staining of A549 using 12108-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRIM21 antibody (12108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 12108-1-AP

    IF Staining of HepG2 using 12108-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TRIM21 antibody (12108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, A549 cells, HeLa cells, HL-60 cells, HT-1080 cells, HT-29 cells, MCF-7 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman pancreas cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy TRIM21 Ready-To-Use IHC Kit. TRIM21 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12108-1-AP targets TRIM21 in WB, IP, IF, IHC, coIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IF/ICC, IHC, ELISA
Cited ApplicationsWB, IP, IF, IHC, coIP
Tested Reactivity human
Cited Reactivityhuman, mouse, hamster, pig
Immunogen TRIM21 fusion protein Ag2753 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 21
Synonyms 52 kDa Ro protein, RING finger protein 81, RNF81, Ro(SS A), RO52, SS A, SSA, SSA1, TRIM21, tripartite motif containing 21
Calculated Molecular Weight 475 aa, 54 kDa
Observed Molecular Weight 42-45 kDa, 49-54 kDa
GenBank Accession NumberBC010861
Gene Symbol TRIM21
Gene ID (NCBI) 6737
RRIDAB_2209469
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19474
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules. TRIM21 exists various isoforms and range molecular weight of isoforms is about 42-45kDa and 49-54 kDa.

实验方案

Product Specific Protocols
WB protocol for TRIM21 antibody 12108-1-APDownload protocol
IHC protocol for TRIM21 antibody 12108-1-APDownload protocol
IF protocol for TRIM21 antibody 12108-1-APDownload protocol
IP protocol for TRIM21 antibody 12108-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

J Clin Invest

The ubiquitin ligase TRIM21 regulates mutant p53 accumulation and gain-of-function in cancer

Authors - Juan Liu
View Article
humanWB

Autophagy

Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules

Authors - Cuiwei Yang
  • KD Validated
View Article
humanWB,IP

Nat Commun

Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.

Authors - Jiawei Zhang
View Article
mouseIF

Transl Res

MECHANISM OF ACTION OF IC 100, A HUMANIZED IgG4 MONOCLONAL ANTIBODY TARGETING APOPTOSIS-ASSOCIATED SPECK-LIKE STAINING PROTEIN CONTAINING A CASPASE RECRUITMENT DOMAIN (ASC).

Authors - Juan Pablo de Rivero Vaccari
View Article
humanWB

Proc Natl Acad Sci U S A

Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 protein.

Authors - Jack Chun-Chieh Hsu
View Article
humanWB,coIP

EMBO Rep

TRIM21-mediated proteasomal degradation of SAMHD1 regulates its antiviral activity.

Authors - Zhaolong Li
  • KO Validated
View Article