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TRIM2 Polyclonal antibody

TRIM2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20356-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

E3 ubiquitin-protein ligase TRIM2, EC:2.3.2.27, KIAA0517, RING-type E3 ubiquitin transferase TRIM2, RNF86

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inmouse brain tissue, mouse small intestine tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human kidney tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20356-1-AP targets TRIM2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag14208

Product name: Recombinant human TRIM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC011052

Sequence: MASEGTNIPSPVVRQIDKQFLICSICLERYKNPKVLPCLHTFCERCLQNYIPAHSLTLSCPVCRQTSILPEKGVAALQNNFFITNLMDVLQRTPGSNAEESSILETVTAVAAGKPLSCPNHDGNVMEFYCQSCETAMCRECTEGEHAEHPTVPLKDVVEQHKASLQVQLDAVNKRLPEIDSALQFISEIIHQLTNQKASIVDDIHSTFDELQKTLNVRKSVLLMELEVNYGLKHKVLQSQLDTLLQGQESIKSCSNFTAQALNHGTETEVLLVKKQMSEKLNELADQDFPLHPRENDQLD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 2
别名 E3 ubiquitin-protein ligase TRIM2, EC:2.3.2.27, KIAA0517, RING-type E3 ubiquitin transferase TRIM2, RNF86
计算分子量 744 aa, 82 kDa
观测分子量 70-85 kDa
GenBank蛋白编号BC011052
基因名称 TRIM2
Gene ID (NCBI) 23321
RRIDAB_10734319
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9C040
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tripartite motif-containing 2(TRIM2) is an E3 ubiquitin ligase which directs proteasome-mediated degradation of target proteins by the ubiquitination of NEFL and phosphorylation of BCL2L11. This protein contains an N-terminal RING domain, followed by a B-box-2 domain, a coiled-coil region, and 6 C-terminal NHL repeats. TRIM2 involves in the apoptotic response to ischemia via directing degradation of BIM protein, thus has a role in neuroprotection.

实验方案

Product Specific Protocols
WB protocol for TRIM2 antibody 20356-1-APDownload protocol
IHC protocol for TRIM2 antibody 20356-1-APDownload protocol
IF protocol for TRIM2 antibody 20356-1-APDownload protocol
IP protocol for TRIM2 antibody 20356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP,IF

Nat Commun

Divergent self-association properties of paralogous proteins TRIM2 and TRIM3 regulate their E3 ligase activity

Authors - Diego Esposito
humanWB,IF

J Transl Med

Stress-induced epinephrine promotes epithelial-to-mesenchymal transition and stemness of CRC through the CEBPB/TRIM2/P53 axis.

Authors - Zili Zhou
humanWB

Cancer Cell Int

TRIM2 directly deubiquitinates and stabilizes Snail1 protein, mediating proliferation and metastasis of lung adenocarcinoma.

Authors - Zhaoxian Lin
  • KD Validated
humanWB,IP,IF

FEBS J

TRIM2 promotes metabolic adaptation to glutamine deprivation via enhancement of CPT1A activity

Authors - Kaimin Liao
humanWB,IHC

Cell Mol Life Sci

E3 ubiquitin ligase TRIM2 identified as a novel suppressor of CYP11B2 and aldosterone production

Authors - Liang Chen
  • KD Validated
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