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验证数据展示

  • WB analysis of Jurkat using 22021-1-AP

    WB analysis of Jurkat using 22021-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22021-1-AP (RFP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 22021-1-AP

    IHC staining of human breast cancer using 22021-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22021-1-AP (RFP2 antibody at dilution of 1:50.

  • IHC staining of human breast cancer using 22021-1-AP

    IHC staining of human breast cancer using 22021-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22021-1-AP (RFP2 antibody at dilution of 1:50.

  • IP experiment of Jurkat using 22021-1-AP

    IP experiment of Jurkat using 22021-1-AP

    IP result of anti-RFP2 (IP:22021-1-AP, 4ug; Detection:22021-1-AP 1:1000) with Jurkat cells lysate 3200ug.

RFP2 Polyclonal antibody

RFP2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 22021-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA and More (1)

别名

CAR, DLEU5, LEU5, Leukemia associated protein 5, Putative tumor suppressor RFP2, Ret finger protein 2, RFP2, RING finger protein 77, RNF77, TRIM13, tripartite motif containing 13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

22021-1-AP targets RFP2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性human
免疫原 RFP2 fusion protein Ag16805 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 13
别名 CAR, DLEU5, LEU5, Leukemia associated protein 5, Putative tumor suppressor RFP2, Ret finger protein 2, RFP2, RING finger protein 77, RNF77, TRIM13, tripartite motif containing 13
计算分子量 407 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC003579
基因名称 RFP2/TRIM13
Gene ID (NCBI) 10206
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60858
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

发表文章

SpeciesApplicationTitle
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss
View Article