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RFP2 Polyclonal antibody

RFP2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 22021-1-AP

Print datasheet

Synonyms

CAR, DLEU5, LEU5, Leukemia associated protein 5, Putative tumor suppressor RFP2, Ret finger protein 2, RFP2, RING finger protein 77, RNF77, TRIM13, tripartite motif containing 13

验证数据展示

  • WB analysis of Jurkat using 22021-1-AP

    WB analysis of Jurkat using 22021-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22021-1-AP (RFP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 22021-1-AP

    IHC staining of human breast cancer using 22021-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22021-1-AP (RFP2 antibody at dilution of 1:50.

  • IHC staining of human breast cancer using 22021-1-AP

    IHC staining of human breast cancer using 22021-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22021-1-AP (RFP2 antibody at dilution of 1:50.

  • IP experiment of Jurkat using 22021-1-AP

    IP experiment of Jurkat using 22021-1-AP

    IP result of anti-RFP2 (IP:22021-1-AP, 4ug; Detection:22021-1-AP 1:1000) with Jurkat cells lysate 3200ug.

经过测试的应用

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

The immunogen of 22021-1-AP is RFP2 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen RFP2 fusion protein Ag16805 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 13
Synonyms CAR, DLEU5, LEU5, Leukemia associated protein 5, Putative tumor suppressor RFP2, Ret finger protein 2, RFP2, RING finger protein 77, RNF77, TRIM13, tripartite motif containing 13
Calculated Molecular Weight 407 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC003579
Gene Symbol TRIM13
Gene ID (NCBI) 10206
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60858
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

发表文章

SpeciesApplicationTitle
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss
View Article