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验证数据展示

  • WB analysis using 27899-1-AP

    WB analysis using 27899-1-AP

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 27899-1-AP (TRIB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 27899-1-AP

    IHC staining of human prostate cancer using 27899-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27899-1-AP (TRIB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRIB1 Polyclonal antibody

TRIB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27899-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C8FW, GIG 2, GIG2, GIG-2, G-protein-coupled receptor-induced gene 2 protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMDA-MB-231 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27899-1-AP targets TRIB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag27541

Product name: Recombinant human TRIB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-150 aa of BC063292

Sequence: MRVGPVRSAMSGASQPRGPALLFPATRGVPAKRLLDADDAAAVAAKCPRLSECSSPPDYLSPPGSPCSPQPPPAAPGAGGGSGSAPGPSRIADYLLLPLAEREHVSRALCIHTGRELRCKVFPIKHYQDKIRPYIQLPSHSNITGIVEVI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tribbles homolog 1 (Drosophila)
别名 C8FW, GIG 2, GIG2, GIG-2, G-protein-coupled receptor-induced gene 2 protein
计算分子量 372 aa, 41 kDa
观测分子量41 kDa
GenBank蛋白编号BC063292
基因名称 TRIB1
Gene ID (NCBI) 10221
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RU8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIB1, a pseudokinase encoded by the tribbles homolog 1 gene, regulates hepatic lipogenesis and lipid metabolism by modulating C/EBPα degradation, thereby influencing plasma lipid profiles and atherosclerotic risk. It is expressed in macrophages and vascular smooth muscle cells, linking metabolic and inflammatory pathways in cardiometabolic disorders (PMID: 26517927, PMID: 40331689).

实验方案

Product Specific Protocols
IHC protocol for TRIB1 antibody 27899-1-APDownload protocol
WB protocol for TRIB1 antibody 27899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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