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验证数据展示

  • WB analysis using 21226-1-AP

    WB analysis using 21226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21226-1-AP (TRHDE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

TRHDE Polyclonal antibody

TRHDE Polyclonal Antibody for WB, ELISA
Cat No. 21226-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

TRH degrading ectoenzyme, TRH DE, Thyroliberinase, Pyroglutamyl peptidase II, PGPEP2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21226-1-AP targets TRHDE in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15681

Product name: Recombinant human TRHDE protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 675-1024 aa of BC150181

Sequence: KGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNIFNEYILKQVATTYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLNRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTRYGEALFMNSKLISGVTEFLNTEGELKELKNFMKNYDGVAAASFSRAVETVEANVRWKMLYQDELFQWLGKALRH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thyrotropin-releasing hormone degrading enzyme
别名 TRH degrading ectoenzyme, TRH DE, Thyroliberinase, Pyroglutamyl peptidase II, PGPEP2
计算分子量 1024 aa, 117 kDa
观测分子量122 kDa
GenBank蛋白编号BC150181
基因名称 TRHDE
Gene ID (NCBI) 29953
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKU6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thyrotropin-releasing hormone-degrading ectoenzyme (TRHDE) belongs to the peptidase M1 family. TRHDE expression is detected at relatively late phases of brain development. TRHDE functions to inactivate thyrotropin-releasing hormone (PMID: 30518145, PMID: 32457627).

实验方案

Product Specific Protocols
WB protocol for TRHDE antibody 21226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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