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验证数据展示

  • WB analysis using 68723-1-Ig (same clone as 68723-1-PBS)

    WB analysis using 68723-1-Ig (same clone as 68723-1-PBS)

    Non-treated and PMA treated THP-1 cellls were subjected to SDS PAGE followed by western blot with 68723-1-Ig (TREM2 antibody) at dilution of 1:5000 incubated at room temperature for 1 hours. This data was developed using the same antibody clone with 68723-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 68723-1-Ig (same clone as 68723-1-PBS)

    IF Staining of mouse brain using 68723-1-Ig (same clone as 68723-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TREM2 antibody (68723-1-Ig, Clone: 1F4H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68723-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 68723-1-Ig (same clone as 68723-1-PBS)

    IF Staining of rat brain using 68723-1-Ig (same clone as 68723-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using TREM2 antibody (68723-1-Ig, Clone: 1F4H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68723-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 68723-1-Ig (same clone as 68723-1-PBS)

    IF Staining of rat brain using 68723-1-Ig (same clone as 68723-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using TREM2 antibody (68723-1-Ig, Clone: 1F4H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68723-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 68723-1-Ig (same clone as 68723-1-PBS)

    IF Staining of mouse brain using 68723-1-Ig (same clone as 68723-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TREM2 antibody (68723-1-Ig, Clone: 1F4H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68723-1-PBS in a different storage buffer formulation.

TREM2 Monoclonal antibody, PBS Only

TREM2 Monoclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 68723-1-PBS

产品说明书

CloneNo. 1F4H10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF-P, Indirect ELISA

别名

Trem2c, Trem2b, Trem2a, TREM-2, TREM 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TREM2 Ready-To-Use IHC Kit. TREM2 primary antibody included.

产品信息

68723-1-PBS targets TREM2 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TREM2 fusion protein Ag6843 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 triggering receptor expressed on myeloid cells 2
别名 Trem2c, Trem2b, Trem2a, TREM-2, TREM 2
计算分子量 222 aa, 25 kDa
观测分子量26-32 kDa
GenBank蛋白编号BC032362
基因名称 TREM2
Gene ID (NCBI) 54209
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NZC2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213). TREM2 has a calculated molecular mass of ~25kDa, but apparent molecular mass of ~40kDa (PMID: 24078628). In addition, TREM2 has a soluble form termed as sTREM-2 with molecular weights ranging between about 24kDa and 40kDa (PMID: 18790823). Loss-of-function mutations in either TREM2 or DAP12 can cause Nasu-Hakola disease (PMID: 19302484).