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验证数据展示

  • WB analysis of mouse thymus using 13279-1-AP

    WB analysis of mouse thymus using 13279-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13279-1-AP (TRAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 13279-1-AP

    IP experiment of Jurkat using 13279-1-AP

    IP result of anti-TRAT1 (IP:13279-1-AP, 4ug; Detection:13279-1-AP 1:300) with Jurkat cells lysate 3000 ug.

  • IHC staining of human tonsillitis using 13279-1-AP

    IHC staining of human tonsillitis using 13279-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13279-1-AP (TRAT1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 13279-1-AP

    IHC staining of human tonsillitis using 13279-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13279-1-AP (TRAT1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRAT1 Polyclonal antibody

TRAT1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13279-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

HSPC062, pp29/30, TCRIM, TRAT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse thymus tissue, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13279-1-AP targets TRAT1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TRAT1 fusion protein Ag4023 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 T cell receptor associated transmembrane adaptor 1
别名 HSPC062, pp29/30, TCRIM, TRAT1
计算分子量 186 aa, 21 kDa
观测分子量 29 kDa
GenBank蛋白编号BC025713
基因名称 TRAT1
Gene ID (NCBI) 50852
RRIDAB_10643524
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6PIZ9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TRAT1 antibody 13279-1-APDownload protocol
IHC protocol for TRAT1 antibody 13279-1-APDownload protocol
IP protocol for TRAT1 antibody 13279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols