验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, human brain tissue, mouse kidney tissue |
| Positive IHC detected in | human heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19549-1-AP targets TRAPPC9/NIBP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | trafficking protein particle complex 9 |
| 别名 | IBP, IKBKBBP, KIAA1882, NIBP, NIK and IKBKB binding protein, T1, TRAPPC9, TRS120, Tularik gene 1 protein |
| 计算分子量 | 139 kDa |
| 观测分子量 | 56 kDa |
| GenBank蛋白编号 | NM_031466 |
| 基因名称 | NIBP/Trappc9 |
| Gene ID (NCBI) | 83696 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96Q05 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRAPPC9, also named NIK- and IKBKB-binding protein (NIBP), is involved in the NF-kappaB signaling pathway and directly interacts with IKK-beta and MAP3K14. TRAPPC9 functions as an activator of NF-kappa-B via increased phosphorylation of the IKK complex. TARPPC9 may also function in neuronal cells differentiation and defects in TRAPPC9 are the cause of mental retardation autosomal recessive type 13 (MRT13), which is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior. TARPPC9 is a component of the multisubunit TRAPP (transport protein particle) complex and it may play a role in vesicular transport from endoplasmic reticulum to Golgi.
