验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20752-1-AP targets TRAPPC5 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | TRAPPC5 fusion protein Ag13185 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | trafficking protein particle complex 5 |
| 别名 | TRAPPC 5, Trafficking protein particle complex subunit 5 |
| 计算分子量 | 188 aa, 21 kDa |
| 观测分子量 | 21-23 kDa |
| GenBank蛋白编号 | BC042161 |
| 基因名称 | TRAPPC5 |
| Gene ID (NCBI) | 126003 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IUR0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC5 is a component of the TRAPP complexes and is involved in processes such as intracellular vesicle transport. In hepatocellular carcinoma, upregulated expression of TRAPPC5 can enhance cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) and promote tumorigenesis (PMID: 35425722).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAPPC5 antibody 20752-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |