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验证数据展示

  • WB analysis using 84119-3-RR

    WB analysis using 84119-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84119-3-RR (TRAPPC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 84119-3-RR

    WB analysis of MCF-7 using 84119-3-RR

    WB result of TRAPPC4 antibody (84119-3-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC4 transfected MCF-7 cells.

  • IP experiment of HL-60 using 84119-3-RR

    IP experiment of HL-60 using 84119-3-RR

    IP result of anti-TRAPPC4 (IP:84119-3-RR, 4ug; Detection:84119-3-RR 1:3000) with HL-60 cells lysate 2240 ug.

  • IHC staining of human colon cancer using 84119-3-RR

    IHC staining of human colon cancer using 84119-3-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84119-3-RR (TRAPPC4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84119-3-RR

    IF Staining of HeLa using 84119-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAPPC4 antibody (84119-3-RR, Clone: 241158C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84119-3-RR

    Affinity and Kinetic Characterization of 84119-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84119-3-RR against Human TRAPPC4 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

TRAPPC4 Recombinant antibody

TRAPPC4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 84119-3-RR

产品说明书

CloneNo. 241158C11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

241158C11, CGI 104, CGI-104, HSPC172, PTD009

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHCT 116 cells, MCF-7 cells, HL-60 cells, RAW 264.7 cells, mouse testis tissue, rat testis tissue
Positive IP detected inHL-60 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84119-3-RR targets TRAPPC4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TRAPPC4 fusion protein Ag2737 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 trafficking protein particle complex 4
别名 241158C11, CGI 104, CGI-104, HSPC172, PTD009
计算分子量 219 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC010866
基因名称 TRAPPC4
Gene ID (NCBI) 51399
RRIDAB_3671682
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y296
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC4, also known as synbindin, is a core component of the TRAPP complexes and one of the essential subunits for guanine nucleotide exchange factor activity for Rab1 GTPase (PMID: 31794024). Deficiencies in vesicular transport mediated by TRAPPC4 have been associated with severe syndromic intellectual disability (PMID: 31794024).

实验方案

Product Specific Protocols
WB protocol for TRAPPC4 antibody 84119-3-RRDownload protocol
IF protocol for TRAPPC4 antibody 84119-3-RRDownload protocol
IP protocol for TRAPPC4 antibody 84119-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols