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验证数据展示

  • WB analysis using 12074-1-AP

    WB analysis using 12074-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12074-1-AP (TRAPPC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HL-60 using 12074-1-AP

    IP experiment of HL-60 using 12074-1-AP

    IP result of anti-TRAPPC4 (IP:12074-1-AP, 4ug; Detection:12074-1-AP 1:800) with HL-60 cells lysate 1720 ug.

  • IHC staining of human colon cancer using 12074-1-AP

    IHC staining of human colon cancer using 12074-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 12074-1-AP

    IHC staining of human colon cancer using 12074-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 12074-1-AP

    IF Staining of HeLa using 12074-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAPPC4 antibody (12074-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of MCF-7 using 12074-1-AP

    WB analysis of MCF-7 using 12074-1-AP

    WB result of TRAPPC4 antibody (12074-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC4 transfected MCF-7 cells.

  • IHC staining of mouse liver using 12074-1-AP

    IHC staining of mouse liver using 12074-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12074-1-AP (TRAPPC4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

TRAPPC4 Polyclonal antibody

TRAPPC4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12074-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

CGI 104, CGI-104, HSPC172, PTD009, SBDN

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, MCF-7 cells, mouse testis tissue, rat testis tissue
Positive IP detected inHL-60 cells
Positive IHC detected inhuman colon cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12074-1-AP targets TRAPPC4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag2737

Product name: Recombinant human TRAPPC4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-219 aa of BC010866

Sequence: MAIFSVYVVNKAGGLIYQLDSYAPRAEAEKTFSYPLDLLLKLHDERVLVAFGQRDGIRVGHAVLAINGMDVNGRYTADGKEVLEYLGNPANYPVSIRFGRPRLTSNEKLMLASMFHSLFAIGSQLSPEQGSSGIEMLETDTFKLHCYQTLTGIKFVVLADPRQAGIDSLLRKIYEIYSDFALKNPFYSLEMPIRCELFDQNLKLALEVAEKAGTFGPGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trafficking protein particle complex 4
别名 CGI 104, CGI-104, HSPC172, PTD009, SBDN
计算分子量 219 aa, 24 kDa
观测分子量 24-27 kDa
GenBank蛋白编号BC010866
基因名称 TRAPPC4
Gene ID (NCBI) 51399
RRIDAB_2208147
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y296
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC4, also known as synbindin, is a core component of the TRAPP complexes and one of the essential subunits for guanine nucleotide exchange factor activity for Rab1 GTPase (PMID: 31794024). Deficiencies in vesicular transport mediated by TRAPPC4 have been associated with severe syndromic intellectual disability (PMID: 31794024).

实验方案

Product Specific Protocols
IF protocol for TRAPPC4 antibody 12074-1-APDownload protocol
IHC protocol for TRAPPC4 antibody 12074-1-APDownload protocol
IP protocol for TRAPPC4 antibody 12074-1-APDownload protocol
WB protocol for TRAPPC4 antibody 12074-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Res

TRAPPC4 Promotes GPX4 Stability to Drive Ferroptosis Resistance and Tumor Progression in Head and Neck Squamous Cell Carcinoma

Authors - Zhao Ding
View Article
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