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验证数据展示

  • WB analysis using 12074-1-AP

    WB analysis using 12074-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12074-1-AP (TRAPPC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HL-60 using 12074-1-AP

    IP experiment of HL-60 using 12074-1-AP

    IP result of anti-TRAPPC4 (IP:12074-1-AP, 4ug; Detection:12074-1-AP 1:800) with HL-60 cells lysate 1720 ug.

  • IHC staining of human colon cancer using 12074-1-AP

    IHC staining of human colon cancer using 12074-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 12074-1-AP

    IHC staining of human colon cancer using 12074-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 12074-1-AP

    IF Staining of HeLa using 12074-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAPPC4 antibody (12074-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of MCF-7 using 12074-1-AP

    WB analysis of MCF-7 using 12074-1-AP

    WB result of TRAPPC4 antibody (12074-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC4 transfected MCF-7 cells.

  • Featured Product
  • KD/KO验证

TRAPPC4 Polyclonal antibody

TRAPPC4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12074-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

CGI 104, CGI-104, HSPC172, PTD009, SBDN

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, MCF-7 cells, mouse testis tissue, rat testis tissue
Positive IP detected inHL-60 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12074-1-AP targets TRAPPC4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2737

Product name: Recombinant human TRAPPC4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-219 aa of BC010866

Sequence: MAIFSVYVVNKAGGLIYQLDSYAPRAEAEKTFSYPLDLLLKLHDERVLVAFGQRDGIRVGHAVLAINGMDVNGRYTADGKEVLEYLGNPANYPVSIRFGRPRLTSNEKLMLASMFHSLFAIGSQLSPEQGSSGIEMLETDTFKLHCYQTLTGIKFVVLADPRQAGIDSLLRKIYEIYSDFALKNPFYSLEMPIRCELFDQNLKLALEVAEKAGTFGPGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trafficking protein particle complex 4
别名 CGI 104, CGI-104, HSPC172, PTD009, SBDN
计算分子量 219 aa, 24 kDa
观测分子量 24-27 kDa
GenBank蛋白编号BC010866
基因名称 TRAPPC4
Gene ID (NCBI) 51399
RRIDAB_2208147
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y296
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC4, also known as synbindin, is a core component of the TRAPP complexes and one of the essential subunits for guanine nucleotide exchange factor activity for Rab1 GTPase (PMID: 31794024). Deficiencies in vesicular transport mediated by TRAPPC4 have been associated with severe syndromic intellectual disability (PMID: 31794024).

实验方案

Product Specific Protocols
IF protocol for TRAPPC4 antibody 12074-1-APDownload protocol
IHC protocol for TRAPPC4 antibody 12074-1-APDownload protocol
IP protocol for TRAPPC4 antibody 12074-1-APDownload protocol
WB protocol for TRAPPC4 antibody 12074-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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