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TRAPPC2L Polyclonal antibody

TRAPPC2L Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25282-1-AP

Print datasheet

Synonyms

HSPC176, TRAPPC2L

验证数据展示

  • WB analysis using 25282-1-AP

    WB analysis using 25282-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25282-1-AP (TRAPPC2L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 25282-1-AP

    IHC staining of human kidney using 25282-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25282-1-AP (TRAPPC2L Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 25282-1-AP

    IF Staining of HepG2 using 25282-1-AP

    Immunofluorescent analysis of HepG2 cells using 25282-1-AP (TRAPPC2L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, U-251 cells, NIH/3T3 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25282-1-AP is TRAPPC2L Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen TRAPPC2L fusion protein Ag16890 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name trafficking protein particle complex 2-like
Synonyms HSPC176, TRAPPC2L
Calculated Molecular Weight 140 aa, 16 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC105809
Gene Symbol TRAPPC2L
Gene ID (NCBI) 51693
RRIDAB_2880006
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UL33
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TRAPPC2L antibody 25282-1-APDownload protocol
IHC protocol for TRAPPC2L antibody 25282-1-APDownload protocol
IF protocol for TRAPPC2L antibody 25282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols