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验证数据展示

  • WB analysis using 27774-1-AP

    WB analysis using 27774-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27774-1-AP (TRAPPC11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 27774-1-AP

    IP experiment of HeLa using 27774-1-AP

    IP result of anti-TRAPPC11 (IP:27774-1-AP, 4ug; Detection:27774-1-AP 1:500) with HeLa cells lysate 1320 ug.

  • IHC staining of human intrahepatic cholangiocarcinoma using 27774-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 27774-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27774-1-AP (TRAPPC11 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 27774-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 27774-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27774-1-AP (TRAPPC11 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRAPPC11 Polyclonal antibody

TRAPPC11 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 27774-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

C4orf41, Trafficking protein particle complex subunit 11, UPF0636 protein C4orf41

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, U2OS cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27774-1-AP targets TRAPPC11 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag27096

Product name: Recombinant human C4orf41 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 519-735 aa of BC022185

Sequence: LLGRASTLKDDQKSRIEKNLINVLMNESPDPEPDCDILAVKTAQKLWADRISLAGSNIFTIGVQDFVPFVQCKAKFHAPSFHVDVPVQFDIYLKADCPHPIRFSKLCVSFNNQEYNQFCVIEEASKANEVLENLTQGKMCLVPGKTRKLLFKFVAKTEDVGKKIEITSVDLALGNETGRCVVLNWQGGGGDAASSQEALQAARSFKRRPKLPDNEVH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 4 open reading frame 41
别名 C4orf41, Trafficking protein particle complex subunit 11, UPF0636 protein C4orf41
观测分子量129 kDa
GenBank蛋白编号BC022185
基因名称 TRAPPC11
Gene ID (NCBI) 60684
RRIDAB_3085995
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z392
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAPPC11 is a subunit of the TRAPP (transport protein particle) tethering complex, which functions in intracellular vesicle trafficking. This subunit is involved in early stage endoplasmic reticulum-to-Golgi vesicle transport. Mutations in TRAPPC11 result in neuromuscular and developmental phenotypes.

实验方案

Product Specific Protocols
IHC protocol for TRAPPC11 antibody 27774-1-APDownload protocol
IP protocol for TRAPPC11 antibody 27774-1-APDownload protocol
WB protocol for TRAPPC11 antibody 27774-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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