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验证数据展示

  • IF Staining of HeLa using 13868-1-AP

    IF Staining of HeLa using 13868-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAPPC1 antibody (13868-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • WB analysis using 13868-1-AP

    WB analysis using 13868-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13868-1-AP (TRAPPC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13868-1-AP

    IHC staining of mouse brain using 13868-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13868-1-AP (TRAPPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13868-1-AP

    IHC staining of mouse brain using 13868-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13868-1-AP (TRAPPC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRAPPC1 Polyclonal antibody

TRAPPC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13868-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Trafficking protein particle complex subunit 1, MUM-2, MUM2, Multiple myeloma protein 2, BET5 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13868-1-AP targets TRAPPC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TRAPPC1 fusion protein Ag4616 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trafficking protein particle complex 1
别名 Trafficking protein particle complex subunit 1, MUM-2, MUM2, Multiple myeloma protein 2, BET5 homolog
计算分子量 145 aa, 17 kDa
观测分子量 15-17 kDa
GenBank蛋白编号BC032717
基因名称 TRAPPC1
Gene ID (NCBI) 58485
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5R8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAPPC1, also known as BET5 or MUM2, is a part of the multisubunit TRAPP (transport protein particle) complexes which are involved in the tethering process at different trafficking steps of vesicle transport (PMID: 16828797). TRAPPC1 may play a role in vesicular transport from the endoplasmic reticulum to the Golgi (PMID: 10582700).