验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, HeLa cells, mouse heart tissue, Raji cells, HepG2 cells, K-562 cells, NIH/3T3 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10325-1-AP targets TRAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag0400 Product name: Recombinant human TRAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 446-704 aa of BC000406 Sequence: MREGIVTATEQEVKEDIAKLLRYESSALPSGQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKALERH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TNF receptor-associated protein 1 |
| 别名 | Heat shock protein family C member 5, HSP 75, HSP75, HSP90L, HSPC5 |
| 计算分子量 | 80 kDa |
| 观测分子量 | 66-70 kDa, 60 kDa |
| GenBank蛋白编号 | BC000406 |
| 基因名称 | TRAP1 |
| Gene ID (NCBI) | 10131 |
| RRID | AB_2303284 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q12931 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TNF receptor-associated protein 1 (TRAP1, synonym: HSP75) is a member of the HSP90 family of molecular chaperones, and is expressed ubiquitously and located to mitochondria. A unique LxCxE motif in HSP75, but not in other HSP90 family members, appears to be important for binding to the simian virus 40 T-antigen-binding domain of hypophosphorylated Rb. Data also suggests that suppression of the expression of TRAP1 in mitochondria plays an important role in the induction of apoptosis caused via formation of reactive oxygen species (ROS).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAP1 antibody 10325-1-AP | Download protocol |
| IHC protocol for TRAP1 antibody 10325-1-AP | Download protocol |
| IF protocol for TRAP1 antibody 10325-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Nanotechnol An orally administered gene editing nanoparticle boosts chemo-immunotherapy in colorectal cancer | ||
Nat Immunol Cancer suppresses mitochondrial chaperone activity in macrophages to drive immune evasion | ||
Mol Cell Deciphering functional tumor-immune crosstalk through highly multiplexed imaging and deep visual proteomics | ||
J Control Release Acid neutralization and metal-phenolic nanomedicine on-demand release microspheres for reversing cartilage degeneration. | ||
Apoptosis Ochratoxin A-induced mitochondrial pathway apoptosis and ferroptosis by promoting glycolysis | ||
Cancer Cell Int Stress granules affect the dual PI3K/mTOR inhibitor response by regulating the mitochondrial unfolded protein response |
