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验证数据展示

  • WB analysis using 27064-1-AP (same clone as 27064-1-PBS)

    WB analysis using 27064-1-AP (same clone as 27064-1-PBS)

    Human milk sample were subjected to SDS PAGE followed by western blot with 27064-1-AP (TRAIL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 27064-1-AP (same clone as 27064-1-PBS)

    IHC staining of human kidney using 27064-1-AP (same clone as 27064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 27064-1-AP (same clone as 27064-1-PBS)

    IHC staining of human kidney using 27064-1-AP (same clone as 27064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 27064-1-AP (same clone as 27064-1-PBS)

    IF Staining of MCF-7 using 27064-1-AP (same clone as 27064-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27064-1-AP (TRAIL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TRAIL/CD253 Polyclonal antibody, PBS Only

TRAIL/CD253 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27064-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

TNFSF10, TRAIL, Apo-2 ligand, CD253, Protein TRAIL

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

27064-1-PBS targets TRAIL/CD253 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 TRAIL/CD253 fusion protein Ag25746 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor necrosis factor (ligand) superfamily, member 10
别名 TNFSF10, TRAIL, Apo-2 ligand, CD253, Protein TRAIL
计算分子量 281 aa, 33 kDa
观测分子量 33 kDa, 20 kDa
GenBank蛋白编号BC032722
基因名称 TRAIL
Gene ID (NCBI) 8743
RRIDAB_2880737
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50591
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.