TRAF7 Polyclonal antibody

TRAF7 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA and More (2)

Conjugate

Unconjugated

Cat No : 11780-1-AP

Print datasheet

Synonyms

RFWD1, RING finger protein 119, RNF119, TRAF7



经过测试的应用

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11780-1-AP targets TRAF7 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen TRAF7 fusion protein Ag2414 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TNF receptor-associated factor 7
Synonyms RFWD1, RING finger protein 119, RNF119, TRAF7
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 67-75 kDa
GenBank Accession NumberBC024267
Gene Symbol TRAF7
Gene ID (NCBI) 84231
RRIDAB_2877793
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6Q0C0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TRAF7 antibody 11780-1-APDownload protocol
IHC protocol for TRAF7 antibody 11780-1-APDownload protocol
IP protocol for TRAF7 antibody 11780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse, humanIF

Kidney Int

Copy number variation analysis identifies novel CAKUT candidate genes in children with a solitary functioning kidney.

Authors - Rik Westland
humanWB

Mol Ther Nucleic Acids

HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis.

Authors - Zhi Zhang
humanWB

Virol Sin

TRAF7 negatively regulates the RLR signaling pathway by facilitating the K48-linked ubiquitination of TBK1

Authors - Jing-Ping Huang
  • KO Validated
humanWB,CoIP

Cell Death Discov

TRAF7-targeted HOXA5 acts as a tumor suppressor in prostate cancer progression and stemness via transcriptionally activating SPRY2 and regulating MEK/ERK signaling

Authors - Jianfeng Ye
mouse,ratWB

Free Radic Biol Med

Hydrogen sulfide improves endothelial barrier function by modulating the ubiquitination degradation of KLF4 through TRAF7 S-sulfhydration in diabetic aorta

Authors - Qianzhu Li