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CoraLite®594-conjugated TRAF6 Monoclonal antibody

TRAF6 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D1E1

Cat No : CL594-66498

Print datasheet

Synonyms

RING finger protein 85, RNF85, TRAF6

验证数据展示

  • IF Staining of HeLa using CL594-66498

    IF Staining of HeLa using CL594-66498

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated TRAF6 antibody (CL594-66498, Clone: 1D1E1 ) at dilution of 1:100.

  • FC experiment of Jurkat using CL594-66498

    FC experiment of Jurkat using CL594-66498

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TRAF6 (CL594-66498, Clone:1D1E1) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy TRAF6 Ready-To-Use IHC Kit. TRAF6 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66498 targets TRAF6 in IF, FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse
Immunogen TRAF6 fusion protein Ag24696 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TNF receptor-associated factor 6
Synonyms RING finger protein 85, RNF85, TRAF6
Calculated Molecular Weight 522 aa, 60 kDa
GenBank Accession NumberBC031052
Gene Symbol TRAF6
Gene ID (NCBI) 7189
RRIDAB_2920000
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y4K3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.

实验方案

Product Specific Protocols
IF protocol for CL594 TRAF6 antibody CL594-66498Download protocol
FC protocol for CL594 TRAF6 antibody CL594-66498Download protocol
Standard Protocols
Click here to view our Standard Protocols