验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, PC-3 cells, mouse pancreas tissue, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12868-1-AP targets TRAF5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3519 Product name: Recombinant human TRAF5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 209-557 aa of BC029600 Sequence: DEHLAVCPEAEQDCPFKHYGCAVTDKRRNLQQHEHSALREHMRLVLEKNVQLEEQISDLHKSLEQKESKIQQLAETIKKLEKEFKQFAQLFGKNGSFLPNIQVFASHIDKSAWLEAQVHQLLQMVNQQQNKFDLRPLMEAVDTVKQKITLLENNDQRLAVLEEETNKHDTHINIHKAQLSKNEERFKLLEGTCYNGKLIWKVTDYKMKKREAVDGHTVSIFSQSFYTSRCGYRLCARAYLNGDGSGRGSHLSLYFVVMRGEFDSLLQWPFRQRVTLMLLDQSGKKNIMETFKPDPNSSSFKRPDGEMNIASGCPRFVAHSVLENAKNAYIKDDTLFLKVAVDLTDLEDL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TNF receptor-associated factor 5 |
| 别名 | RING finger protein 84, RNF84, TRAF5 |
| 计算分子量 | 557 aa, 64 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC029600 |
| 基因名称 | TRAF5 |
| Gene ID (NCBI) | 7188 |
| RRID | AB_2209712 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00463 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAF5 antibody 12868-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Neuroinflammation CD36 deletion prevents white matter injury by modulating microglia polarization through the Traf5-MAPK signal pathway | ||
Cancer Lett Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells. | ||
Biochim Biophys Acta Mol Cell Res IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5 | ||
J Cell Mol Med Down-regulated HDAC3 elevates microRNA-495-3p to restrain epithelial-mesenchymal transition and oncogenicity of melanoma cells via reducing TRAF5.
| ||
Eur J Pharmacol TRAF5 protects against myocardial ischemia reperfusion injury via AKT signaling.
| ||
Med Oncol DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway. |
