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验证数据展示

  • WB analysis using 66755-1-Ig (same clone as 66755-1-PBS)

    WB analysis using 66755-1-Ig (same clone as 66755-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 66755-1-Ig (same clone as 66755-1-PBS)

    IHC staining of human urothelial carcinoma using 66755-1-Ig (same clone as 66755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 66755-1-Ig (same clone as 66755-1-PBS)

    IHC staining of human urothelial carcinoma using 66755-1-Ig (same clone as 66755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66755-1-Ig (same clone as 66755-1-PBS)

    IF Staining of HeLa using 66755-1-Ig (same clone as 66755-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66755-1-Ig (TRAF4 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • WB analysis using 66755-1-Ig (same clone as 66755-1-PBS)

    WB analysis using 66755-1-Ig (same clone as 66755-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TRAF4 Monoclonal antibody, PBS Only

TRAF4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66755-1-PBS

产品说明书

CloneNo. 2C9A11

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CART1, MLN 62, MLN62, RING finger protein 83, RNF83, TRAF4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66755-1-PBS targets TRAF4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, pig
免疫原 TRAF4 fusion protein Ag27221 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 TNF receptor-associated factor 4
别名 CART1, MLN 62, MLN62, RING finger protein 83, RNF83, TRAF4
计算分子量 54 kDa
观测分子量 50-54 kDa
GenBank蛋白编号BC001769
基因名称 TRAF4
Gene ID (NCBI) 9618
RRIDAB_2882101
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BUZ4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The tumor necrosis factor receptor-associated factor (TRAF) family, consisting of seven members (TRAF1-7), is a group of signaling adaptors which participate in various signaling pathways through binding of the tumor necrosis factor receptor superfamily. As one of the important member of TRAF family proteins, TRAF4 was initially isolated from breast carcinomas and identified as the first member of the TRAF family to be up-regulated in human carcinomas. TRAF4 is required during embryogenesis in key biological processes including the formation of the trachea, the development of the axial skeleton, and the closure of the neural tube. TRAF4 deficiency leads to severe developmental alterations.