验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, human colon cancer tissue, mouse thymus tissue |
| Positive IHC detected in | human colon cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 2 publications below |
产品信息
18110-1-AP targets TRAF3IP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag12559 Product name: Recombinant human TRAF3IP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC110302 Sequence: MISPDPRPSPGLARWAESYEAKCERRQEIRESRRCRPNVTTCRQVGKTLRIQQREQLQRARLQQFFRRRNLELEEKGKAQHPQAREQGPSRRPGQVTVLKEPLSCARRISSPREQVTGTSSEVFPAQHPPPSGICRDLSDHLSSQAGGLPPQDTPIKKPPKHHRGTQTKAEGPTIKNDASQQTNYGVAVLDKEIIQLSDYLKEALQRELVLKQKMVILQDLLSTLIQASDSSWKGQLNEDKLKGKLRSLENQLYTCTQKYSPWGMKKVLLEMEDQKNSYEQKAKESLQKVLEEKMNAEQQLQSTQRSLALAEQKCEEWRSQYEALKEDWRTLGTQHRELESQLHVLQSKLQIN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TRAF3 interacting protein 3 |
| 别名 | T3JAM, TRAF3 interacting protein 3, TRAF3IP3 |
| 计算分子量 | 353aa,41 kDa; 551aa,64 kDa |
| 观测分子量 | 64 kDa, 47 kDa, 41 kDa |
| GenBank蛋白编号 | BC110302 |
| 基因名称 | TRAF3IP3 |
| Gene ID (NCBI) | 80342 |
| RRID | AB_2918057 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y228 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRAF3 interacting protein 3 (TRAF3IP3) is an essential adapter protein containing a coiled-coil domain that affects both innate and adaptive immunity. It plays a crucial role in the regulation of thymocyte development (PMID:26195727), and it also regulates T-cell stability and function by recruiting the serine-threonine phosphatase catalytic subunit (PPP2CA) to the lysosome, thereby facilitating the interaction of PP2Ac with the mTORC1 component RPTOR and restricting glycolytic metabolism (PMID:30115741). TRAF3IP3 responses to viral infection, facilitates the recruitment of TRAF3 to MAVS within mitochondria leading to IRF3 activation and interferon production (PMID:31390091). TRAF3IP3 has 3 isoforms produced by alternative splicing with the molecular weight of 64 kDa, 47 kDa and 41 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAF3IP3 antibody 18110-1-AP | Download protocol |
| IHC protocol for TRAF3IP3 antibody 18110-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Braz J Med Biol Res SNORA5A regulates tumor-associated macrophage M1/M2 phenotypes via TRAF3IP3 in breast cancer | ||
Adv Sci (Weinh) TRAF3IP3 Induces ER Stress-Mediated Apoptosis with Protective Autophagy to Inhibit Lung Adenocarcinoma Proliferation |
