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验证数据展示

  • WB analysis of MCF-7 using 87596-1-RR (same clone as 87596-1-PBS)

    WB analysis of MCF-7 using 87596-1-RR (same clone as 87596-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 87596-1-RR (TRAF3IP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87596-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 87596-1-RR (same clone as 87596-1-PBS)

    WB analysis of A549 using 87596-1-RR (same clone as 87596-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 87596-1-RR (TRAF3IP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87596-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 87596-1-RR (same clone as 87596-1-PBS)

    IF Staining of HepG2 using 87596-1-RR (same clone as 87596-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TRAF3IP2 antibody (87596-1-RR, Clone: 252775C5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87596-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03101-1

    Cytometric bead array sample test of MP03101-1

    The mean TRAF3IP2 concentration was determined to be 25.5 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, SH-SY5Y cell extract were served as a negative control.

  • Cytometric bead array standard curve of MP03101-1

    Cytometric bead array standard curve of MP03101-1

    Cytometric bead array standard curve of MP03101-1, TRAF3IP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87596-1-PBS. Detection antibody: 87596-2-PBS. Standard: Ag24840. Range: 0.312-40 ng/mL.

  • Affinity of 87596-1-RR

    Affinity of 87596-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87596-1-RR against Human TRAF3IP2 were performed. The affinity constant is below 1 pM.

TRAF3IP2 Recombinant monoclonal antibody, PBS Only (Capture)

TRAF3IP2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 87596-1-PBS

产品说明书

CloneNo. 252775C5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

ACT1, Adapter protein CIKS, C6orf2, C6orf4, C6orf5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87596-1-PBS targets TRAF3IP2 as part of a matched antibody pair:

MP03101-1: 87596-1-PBS capture and 87596-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag24840

Product name: Recombinant human TRAF3IP2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC002823

Sequence: MNRSIPVEVDESEPYPSQLLKPIPEYSPEEESEPPAPNIRNMAPNSLSAPTMLHNSSGDFSQAHSTLKLANHQRPVSRQVTCLRTQVLEDSEDSFCRRHPGLGKAFPSGCSAVSEPASESVVGALPAEHQFSFMEKRNQWLVSQLSAASPDTGHDSDKSDQSLPNASADSLGGSQEMVQRPQPHRNRAGLDLPTIDTGYDSQPQDVLGIRQLERPLPLTSVCYPQDLPRPLRSREFPQFEPQRYPACAQMLPPNLSPHAPWNYHYHCPGSPDHQVPYGHDYPRAAYQQVIQPALPGQPLPGASVRGLHPVQKVILNYPSPWDQEERPAQRDCSFPGLPRHQDQPHHQPPN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TRAF3 interacting protein 2
别名 ACT1, Adapter protein CIKS, C6orf2, C6orf4, C6orf5
计算分子量 65 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC002823
基因名称 TRAF3IP2
Gene ID (NCBI) 10758
RRIDAB_3745640
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43734
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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