验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IF | See 1 publications below |
产品信息
14404-1-AP targets TRAF3IP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | mouse |
免疫原 | TRAF3IP1 fusion protein Ag5677 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | TNF receptor-associated factor 3 interacting protein 1 |
别名 | MIP-T3, MIPT3, Microtubule-interacting protein associated with TRAF3, Intraflagellar transport protein 54 homolog, Interleukin-13 receptor alpha 1-binding protein 1 |
计算分子量 | 79 kDa |
观测分子量 | 83 kDa |
GenBank蛋白编号 | BC059174 |
基因名称 | TRAF3IP1 |
Gene ID (NCBI) | 26146 |
RRID | AB_2878054 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8TDR0 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRAF3IP1, also known as MIPT3, was initially characterized through its interactions with tubulin, actin, TNFR-associated factor-3 (Traf3), IL-13R1, and DISC1. It functions as an inhibitor of IL-13-mediated phosphorylation of Stat6 and in sequestration of Traf3 and DISC1 to the cytoskeleton.
实验方案
Product Specific Protocols | |
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WB protocol for TRAF3IP1 antibody 14404-1-AP | Download protocol |
IHC protocol for TRAF3IP1 antibody 14404-1-AP | Download protocol |
IF protocol for TRAF3IP1 antibody 14404-1-AP | Download protocol |
IP protocol for TRAF3IP1 antibody 14404-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |