TRAF3IP1 Polyclonal antibody

TRAF3IP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14404-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

MIP-T3, MIPT3, Microtubule-interacting protein associated with TRAF3, Intraflagellar transport protein 54 homolog, Interleukin-13 receptor alpha 1-binding protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14404-1-AP targets TRAF3IP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 TRAF3IP1 fusion protein Ag5677 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TNF receptor-associated factor 3 interacting protein 1
别名 MIP-T3, MIPT3, Microtubule-interacting protein associated with TRAF3, Intraflagellar transport protein 54 homolog, Interleukin-13 receptor alpha 1-binding protein 1
计算分子量 79 kDa
观测分子量 83 kDa
GenBank蛋白编号BC059174
基因名称 TRAF3IP1
Gene ID (NCBI) 26146
RRIDAB_2878054
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TDR0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAF3IP1, also known as MIPT3, was initially characterized through its interactions with tubulin, actin, TNFR-associated factor-3 (Traf3), IL-13R1, and DISC1. It functions as an inhibitor of IL-13-mediated phosphorylation of Stat6 and in sequestration of Traf3 and DISC1 to the cytoskeleton.

实验方案

Product Specific Protocols
WB protocol for TRAF3IP1 antibody 14404-1-APDownload protocol
IHC protocol for TRAF3IP1 antibody 14404-1-APDownload protocol
IF protocol for TRAF3IP1 antibody 14404-1-APDownload protocol
IP protocol for TRAF3IP1 antibody 14404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Int J Mol Sci

SMYD3 Controls Ciliogenesis by Regulating Distinct Centrosomal Proteins and Intraflagellar Transport Trafficking

Authors - Ewud Agborbesong
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