验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
29627-1-AP targets TRA2B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag30637 Product name: Recombinant human TRA2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-50 aa of BC005898 Sequence: MSDSGEQNYGERESRSASRSGSAHGSGKSARHTPARSRSKEDSRRSRSKS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transformer 2 beta homolog (Drosophila) |
| 别名 | Htra2 beta, hTRA2-beta, SFRS10, Splicing factor, arginine/serine-rich 10, SRFS10 |
| 计算分子量 | 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC005898 |
| 基因名称 | TRA2B |
| Gene ID (NCBI) | 6434 |
| RRID | AB_2918335 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62995 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transformer-2 protein homolog beta, also known as TRA2B, is a sequence-specific RNA-binding protein that participates in the control of pre-mRNA splicing. TRA2B can alter pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRA2B antibody 29627-1-AP | Download protocol |
| WB protocol for TRA2B antibody 29627-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
