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- KD/KO Validated
TPX2 Polyclonal antibody
TPX2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, K-562 cells, HeLa cells, SMMC-7721 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human prostate cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells, HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11741-1-AP targets TPX2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | TPX2 fusion protein Ag2334 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | TPX2, microtubule-associated, homolog (Xenopus laevis) |
Synonyms | C20orf1, C20orf2, DIL 2, DIL2, FLS353, GD:C20orf1, HCA519, HCTP4, p100, Protein fls353, REPP86, Targeting protein for Xklp2, TPX2 |
Calculated Molecular Weight | 747 aa, 86 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC020207 |
Gene Symbol | TPX2 |
Gene ID (NCBI) | 22974 |
RRID | AB_2208895 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9ULW0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TPX2 antibody 11741-1-AP | Download protocol |
IHC protocol for TPX2 antibody 11741-1-AP | Download protocol |
IF protocol for TPX2 antibody 11741-1-AP | Download protocol |
IP protocol for TPX2 antibody 11741-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Cell Biol WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin. | ||
EMBO Rep Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis | ||
J Cell Sci Loss of protein phosphatase 6 in oocytes causes failure of meiosis II exit and impaired female fertility. | ||
J Cell Sci Importin α phosphorylation promotes TPX2 activation by GM130 to control astral microtubules and spindle orientation. | ||
Front Physiol Dysfunction of Sister Chromatids Separation Promotes Progression of Hepatocellular Carcinoma According to Analysis of Gene Expression Profiling. |