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验证数据展示

  • WB analysis of human kidney using 66174-1-Ig (same clone as 66174-1-PBS)

    WB analysis of human kidney using 66174-1-Ig (same clone as 66174-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66174-1-Ig (TPSAB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66174-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66174-1-Ig (same clone as 66174-1-PBS)

    IHC staining of human tonsillitis using 66174-1-Ig (same clone as 66174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66174-1-Ig (TPSAB1 antibody) at dilution of 1:5000 (under 10x lens) microwave oven heating mediated antigen retrieved with . This data was developed using the same antibody clone with 66174-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPSAB1 antibody included
  • IHC staining of human tonsillitis using 66174-1-Ig (same clone as 66174-1-PBS)

    IHC staining of human tonsillitis using 66174-1-Ig (same clone as 66174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66174-1-Ig (TPSAB1 antibody) at dilution of 1:5000 (under 40x lens) microwave oven heating mediated antigen retrieved with . This data was developed using the same antibody clone with 66174-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPSAB1 antibody included
  • IHC staining of human tonsillitis using 66174-1-Ig (same clone as 66174-1-PBS)

    IHC staining of human tonsillitis using 66174-1-Ig (same clone as 66174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66174-1-Ig (TPSAB1 antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66174-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPSAB1 antibody included
  • Cytometric bead array standard curve of MP50385-1

    Cytometric bead array standard curve of MP50385-1

    Cytometric bead array standard curve of MP50385-1, TPSAB1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66174-1-PBS. Detection antibody: 66174-2-PBS. Standard:Ag4167. Range: 3.125-200 ng/mL.

TPSAB1 Monoclonal antibody, PBS Only (Capture)

TPSAB1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 66174-1-PBS

产品说明书

CloneNo. 6A10G1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

6A10G1, EC:3.4.21.59, MCP7, TPS1, TPS2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TPSAB1 Ready-To-Use IHC Kit. TPSAB1 primary antibody included.

产品信息

66174-1-PBS targets TPSAB1 as part of a matched antibody pair:

MP50385-1: 66174-1-PBS capture and 66174-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4167

Product name: Recombinant human TPSAB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-275 aa of BC028059

Sequence: MLSLLLLALPVLASPAYAAPAPVQALQQAGIVGGQEAPRSKWPWQVSLRVRDRYWMHFCGGSLIHPQWVLTAAHCLGPDVKDLATLRVQLREQHLYYQDQLLPVSRIIVHPQFYIIQTGADIALLELEEPVNISSRVHTVMLPPASETFPPGMPCWVTGWGDVDNDEPLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIIRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWDEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tryptase alpha/beta 1
别名 6A10G1, EC:3.4.21.59, MCP7, TPS1, TPS2
计算分子量 275 aa, 31 kDa
观测分子量 31 kDa
GenBank蛋白编号BC028059
基因名称 TPSAB1
Gene ID (NCBI) 7177
RRIDAB_2881569
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ15661
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TPSAB1(Tryptase alpha/beta-1) is also named as TPS1, TPS2, TPSB1 and belongs to the peptidase S1 family. It is the major neutral protease present in mast cells and secreted upon the coupled activation-degranulation response of this cell type. TPSAB1 has 2 isoforms produced by alternative splicing with the molecular weight of 31 kDa and 29 kDa. This full length protein has two glycosylation sites.

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