验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, K-562 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human tonsillitis tissue, human appendix tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skin tissue |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | U-937 cells |
Planning an IHC experiment? We recommend our IHCeasy TPSAB1 Ready-To-Use IHC Kit. TPSAB1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13343-1-AP targets TPSAB1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag4167 Product name: Recombinant human TPSAB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-275 aa of BC028059 Sequence: MLSLLLLALPVLASPAYAAPAPVQALQQAGIVGGQEAPRSKWPWQVSLRVRDRYWMHFCGGSLIHPQWVLTAAHCLGPDVKDLATLRVQLREQHLYYQDQLLPVSRIIVHPQFYIIQTGADIALLELEEPVNISSRVHTVMLPPASETFPPGMPCWVTGWGDVDNDEPLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIIRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWDEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tryptase alpha/beta 1 |
| 别名 | EC:3.4.21.59, MCP7, TPS1, TPS2, TPSB1 |
| 计算分子量 | 275 aa, 31 kDa |
| 观测分子量 | 32-38 kDa |
| GenBank蛋白编号 | BC028059 |
| 基因名称 | TPSAB1 |
| Gene ID (NCBI) | 7177 |
| RRID | AB_2206197 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15661 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TPSAB1 antibody 13343-1-AP | Download protocol |
| IHC protocol for TPSAB1 antibody 13343-1-AP | Download protocol |
| IP protocol for TPSAB1 antibody 13343-1-AP | Download protocol |
| WB protocol for TPSAB1 antibody 13343-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Transl Med Integrating single-cell RNA sequencing with spatial transcriptomics reveals an immune landscape of human myometrium during labour | ||
Front Immunol Palmatine treats urticaria by reducing inflammation and increasing autophagy | ||
IUBMB Life Bromodomain-containing protein 9 is a prognostic biomarker associated with immune infiltrates and promotes tumor malignancy through activating notch signaling pathway in negative HIF-2α clear cell renal cell carcinoma. | ||
BMC Cancer The release of tryptase from mast cells promote tumor cell metastasis via exosomes. | ||
Am J Transl Res HucMSC-Ex alleviates DSS-induced colitis in mice by decreasing mast cell activation via the IL-33/ST2 axis | ||
Nat Commun Dysregulated mast cell activation induced by diabetic milieu exacerbates the progression of diabetic peripheral neuropathy in mice |
