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TPRKB Monoclonal antibody

TPRKB Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D5B9

Cat No : 66402-1-Ig

Print datasheet

Synonyms

CGI 121, My019, PRPK binding protein, TP53RK binding protein, TPRKB

验证数据展示

  • WB analysis of HeLa using 66402-1-Ig

    WB analysis of HeLa using 66402-1-Ig

    Western blot analysis of TPRKB in various cell lines using Proteintech antibody 66402-1-Ig at a dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66402-1-Ig

    WB analysis using 66402-1-Ig

    Various cells were subjected to SDS PAGE followed by western blot with 66402-1-Ig (TPRKB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 66402-1-Ig

    IF Staining of Jurkat using 66402-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TPRKB antibody (66402-1-Ig, Clone: 2D5B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, COLO 320 cells, Caco-2 cells, Jurkat cells, Raji cells
Positive IF detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66402-1-Ig targets TPRKB in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human
Immunogen TPRKB fusion protein Ag18770 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TP53RK binding protein
Synonyms CGI 121, My019, PRPK binding protein, TP53RK binding protein, TPRKB
Calculated Molecular Weight 175 aa, 20 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC029492
Gene Symbol TPRKB
Gene ID (NCBI) 51002
RRIDAB_2881776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y3C4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TPRKB, also named as CGI 121, belongs to the CGI121/TPRKB family. It interacts with TP53RK/PRPK and is widely expressed in all tissues.

实验方案

Product Specific Protocols
WB protocol for TPRKB antibody 66402-1-IgDownload protocol
IF protocol for TPRKB antibody 66402-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols