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验证数据展示

  • WB analysis using 83177-5-RR (same clone as 83177-5-PBS)

    WB analysis using 83177-5-RR (same clone as 83177-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83177-5-RR (TPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83177-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 83177-5-RR (same clone as 83177-5-PBS)

    IHC staining of mouse testis using 83177-5-RR (same clone as 83177-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83177-5-RR (TPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83177-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83177-5-RR (same clone as 83177-5-PBS)

    IF Staining of HeLa using 83177-5-RR (same clone as 83177-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TPR antibody (83177-5-RR, Clone: 230413A2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83177-5-PBS in a different storage buffer formulation.

TPR Recombinant monoclonal antibody, PBS Only

TPR Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83177-5-PBS

产品说明书

CloneNo. 230413A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Nucleoprotein TPR, 230413A2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83177-5-PBS targets TPR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 translocated promoter region (to activated MET oncogene)
别名 Nucleoprotein TPR, 230413A2
计算分子量 267 kDa
观测分子量~270 kDa
GenBank蛋白编号NM_003292
基因名称 TPR
Gene ID (NCBI) 7175
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP12270
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nucleoporin TPR was originally identified as the oncogenic activator of the met, raf, and trk protooncogenes. TPR is a large 270 kDa protein with a bipartite structure consisting of a ~1600-residue a-helical coiled-coil N-terminal domain and a highly acidic non-coiled ~800 amino acid carboxy terminus (PMID: 22253824). TPR is localized exclusively to intranuclear filaments associated with the nucleoplasmic side of the NPC, by directly binding to Nup153 (PMID: 12802065).

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