验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, HEK-293 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15057-1-AP targets TPPP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | TPPP3 fusion protein Ag7040 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tubulin polymerization-promoting protein family member 3 |
| 别名 | Tubulin polymerization-promoting protein family member 3, TPPP/p20, p25gamma, p20, CGI-38 |
| 计算分子量 | 19 kDa |
| 观测分子量 | 19-20 kDa |
| GenBank蛋白编号 | BC000691 |
| 基因名称 | TPPP3 |
| Gene ID (NCBI) | 51673 |
| RRID | AB_2878105 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BW30 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TPPP3 (tubulin polymerization promoting protein 3), also named as TPPP/p20, is a new member of the TPPP family which binds tubulin and induces tubulin polymerization and microtubule bundling.TPPP3 has been shown to play an important role in cell proliferation and may be implicated in tumorigenesis and metastasis (19633818). TPPP3 was also identified as specific marker for the connective tissues that envelope tendons due to its distinct expression in the developing tendon sheath (19235716). This antibody detects the 19-20 kDa TPPP3 in HeLa and PC-3 cells. The non-specific bands between 40 and 60 kDa were also occasionally observed.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TPPP3 antibody 15057-1-AP | Download protocol |
| IHC protocol for TPPP3 antibody 15057-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine. | ||
Stem Cell Res Ther Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.
| ||
Front Immunol Integrated analyses of single-cell transcriptomics identify metastasis-associated myeloid subpopulations in breast cancer lung metastasis | ||
Front Dement Quantitative proteomic analysis using a mouse model of Lewy body dementia induced by α-synuclein preformed fibrils injection |
