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验证数据展示

  • WB analysis using 68048-1-Ig

    WB analysis using 68048-1-Ig

    Brain tissues were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human lung using 68048-1-Ig

    IHC staining of human lung using 68048-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPPP antibody included
  • IHC staining of human lung using 68048-1-Ig

    IHC staining of human lung using 68048-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPPP antibody included
  • WB analysis of SH-SY5Y using 68048-1-Ig

    WB analysis of SH-SY5Y using 68048-1-Ig

    WB result of TPPP antibody (68048-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPPP transfected SH-SY5Y cells.

  • IF Staining of Neuro-2a using 68048-1-Ig

    IF Staining of Neuro-2a using 68048-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using TPPP antibody (68048-1-Ig, Clone: 1E5F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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TPPP Monoclonal antibody

TPPP Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68048-1-Ig

产品说明书

CloneNo. 1E5F5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit, chicken

应用

WB, IHC, IF/ICC, ELISA

别名

p25 alpha, p24, EC:3.6.5.-, 25 kDa brain-specific protein, 25 kDa brain specific protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inpig brain tissue, pig cerebellum tissue, rabbit cerebellum tissue, rat cerebellum tissue, SH-SY5Y cells, rabbit brain, rat brain, mouse brain, chicken brain
Positive IHC detected inhuman lung tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNeuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy TPPP Ready-To-Use IHC Kit. TPPP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68048-1-Ig targets TPPP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit, chicken
免疫原

CatNo: Ag19046

Product name: Recombinant human TPPP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-95 aa of BC131506

Sequence: MADKAKPAKAANRTPPKSPGDPSKDRAAKRLSLESEGAGEGAAASPELSALEEAFRRFAVHGDARATGREMHGKNWSKLCKDCQVIDGRNVTVTD

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 tubulin polymerization promoting protein
别名 p25 alpha, p24, EC:3.6.5.-, 25 kDa brain-specific protein, 25 kDa brain specific protein
计算分子量 219 aa, 24 kDa
观测分子量24-29 kDa
GenBank蛋白编号BC131506
基因名称 TPPP
Gene ID (NCBI) 11076
RRIDAB_2918790
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO94811
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TPPP, also named as TPPP1, P24 and P25, belongs to the TPPP family. TPPP promotes in vitro the polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, TPPP inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.

实验方案

Product Specific Protocols
IF protocol for TPPP antibody 68048-1-IgDownload protocol
IHC protocol for TPPP antibody 68048-1-IgDownload protocol
WB protocol for TPPP antibody 68048-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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