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验证数据展示

  • WB analysis of MCF-7 using 25040-1-AP

    WB analysis of MCF-7 using 25040-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25040-1-AP (TPPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 25040-1-AP

    IHC staining of human brain using 25040-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25040-1-AP (TPPP Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with TPPP antibody included
  • IHC staining of human brain using 25040-1-AP

    IHC staining of human brain using 25040-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25040-1-AP (TPPP Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with TPPP antibody included
  • WB analysis using 25040-1-AP

    WB analysis using 25040-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25040-1-AP (TPPP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 25040-1-AP

    WB analysis of SH-SY5Y using 25040-1-AP

    WB result of TPPP antibody (25040-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPPP transfected SH-SY5Y cells.

  • IF Staining of MCF-7 using 25040-1-AP

    IF Staining of MCF-7 using 25040-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TPPP antibody (25040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

TPPP Polyclonal antibody

TPPP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25040-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

25 kDa brain specific protein, p24, p25, p25 alpha, p25alpha, TPPP, TPPP/p25, TPPP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, MCF-7 cells, rat brain tissue
Positive IHC detected inhuman brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy TPPP Ready-To-Use IHC Kit. TPPP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25040-1-AP targets TPPP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TPPP fusion protein Ag19159 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tubulin polymerization promoting protein
别名 25 kDa brain specific protein, p24, p25, p25 alpha, p25alpha, TPPP, TPPP/p25, TPPP1
计算分子量 219 aa, 24 kDa
观测分子量 24-29 kDa
GenBank蛋白编号BC131506
基因名称 TPPP
Gene ID (NCBI) 11076
RRIDAB_2879865
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94811
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TPPP antibody 25040-1-APDownload protocol
IHC protocol for TPPP antibody 25040-1-APDownload protocol
IF protocol for TPPP antibody 25040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols