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KD/KO Validated

TPPII Polyclonal antibody

TPPII Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Publications

Cat No : 14120-1-AP

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Synonyms

TPP 2, TPP II, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, Tripeptidyl peptidase II

验证数据展示

WB analysis of Jurkat using 14120-1-AP
WB result of TPPII antibody (14120-1-AP, 1:1000) with si-control and si-TPPII transfected Jurkat cells.
WB analysis of Jurkat using 14120-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14120-1-AP (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 14120-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14120-1-AP (TPPII antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 14120-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14120-1-AP (TPPII antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14120-1-AP
Immunofluorescent analysis of HepG2 cells, using TPP2 antibody 14120-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP experiment of Jurkat using 14120-1-AP
IP result of anti-TPPII (IP:14120-1-AP, 4ug; Detection:66017-1-Ig, 1:500) with Jurkat cells lysate 2400ug.
FC experiment of HepG2 using 14120-1-AP
1X10^6 HepG2 cells were stained with 0.2ug TPPII antibody (14120-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Cat No. 14120-1-AP

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经过测试的应用

Positive WB detected in	Jurkat cells, Jurkat
Positive IP detected in	Jurkat cells
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells
Positive FC detected in	HepG2 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:20-1:200
Flow Cytometry (FC)	FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 4 publications below
IF	See 2 publications below

产品信息

14120-1-AP targets TPPII in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications	FC, IF/ICC, IHC, IP, WB,ELISA
Cited Applications	WB, IF
Tested Reactivity	human
Cited Reactivity	human
Immunogen	TPPII fusion protein Ag5296 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	tripeptidyl peptidase II
Synonyms	TPP 2, TPP II, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, Tripeptidyl peptidase II
Calculated Molecular Weight	1249 aa, 138 kDa
Observed Molecular Weight	135-140 kDa
GenBank Accession Number	BC039905
Gene Symbol	TPP2
Gene ID (NCBI)	7174
RRID	AB_2206028
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P29144
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).

实验方案

Product Specific Protocols
WB protocol for TPPII antibody 14120-1-AP	Download protocol
IHC protocol for TPPII antibody 14120-1-AP	Download protocol
IF protocol for TPPII antibody 14120-1-AP	Download protocol
IP protocol for TPPII antibody 14120-1-AP	Download protocol
FC protocol for TPPII antibody 14120-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	J Cell Sci Unveiling a novel serpinB2-tripeptidyl peptidase II signaling axis during senescence. Authors - Chia-Li Liao KD Validated View Article
human	WB	J Immunol Cytosolic Processing Governs TAP-Independent Presentation of a Critical Melanoma Antigen. Authors - Nathalie Vigneron View Article
human	IF	Mol Cell Biochem Novel protein-protein interactions of TPPII, p53, and SIRT7. Authors - Jarmila Nahálková View Article
human	WB,IF	Genes Cancer Tripeptidyl Peptidase II Is Required for c-MYC-Induced Centriole Overduplication and a Novel Therapeutic Target in c-MYC-Associated Neoplasms. Authors - Stefan Duensing KD Validated View Article
human	WB	J Immunol The Activation State of CD4 T Cells Alters Cellular Peptidase Activities, HIV Antigen Processing, and MHC Class I Presentation in a Sequence-Dependent Manner. Authors - Julie Boucau View Article

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TPPII Polyclonal antibody

TPPII Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB,ELISA

验证数据展示

WB analysis of Jurkat using 14120-1-AP

WB analysis of Jurkat using 14120-1-AP

IHC staining of human gliomas using 14120-1-AP

IHC staining of human gliomas using 14120-1-AP

IF Staining of HepG2 using 14120-1-AP

IP experiment of Jurkat using 14120-1-AP

FC experiment of HepG2 using 14120-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Unveiling a novel serpinB2-tripeptidyl peptidase II signaling axis during senescence.

Cytosolic Processing Governs TAP-Independent Presentation of a Critical Melanoma Antigen.

Novel protein-protein interactions of TPPII, p53, and SIRT7.

Tripeptidyl Peptidase II Is Required for c-MYC-Induced Centriole Overduplication and a Novel Therapeutic Target in c-MYC-Associated Neoplasms.

The Activation State of CD4 T Cells Alters Cellular Peptidase Activities, HIV Antigen Processing, and MHC Class I Presentation in a Sequence-Dependent Manner.