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验证数据展示

  • WB analysis of human colon using 12479-1-AP

    WB analysis of human colon using 12479-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 12479-1-AP (TPP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 12479-1-AP

    IHC staining of human colon cancer using 12479-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12479-1-AP (TPP1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 12479-1-AP

    IHC staining of human colon cancer using 12479-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12479-1-AP (TPP1 antibody) at dilution of 1:50 (under 40x lens).

TPP1 Polyclonal antibody

TPP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12479-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CLN2, GIG1, LPIC, TPP 1, TPP I, TPP1, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 1, tripeptidyl peptidase I, UNQ267/PRO304

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman colon tissue
Positive IHC detected inhuman colon cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12479-1-AP targets TPP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 TPP1 fusion protein Ag3173 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripeptidyl peptidase I
别名 CLN2, GIG1, LPIC, TPP 1, TPP I, TPP1, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 1, tripeptidyl peptidase I, UNQ267/PRO304
计算分子量 563 aa, 61 kDa
观测分子量 66 kDa
GenBank蛋白编号BC014863
基因名称 TPP1
Gene ID (NCBI) 1200
RRIDAB_2205902
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14773
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIPEPTIDYL PEPTIDASE I, TPP1, is a lysosomal exopeptidase that sequentially removes tripeptides from the N termini of proteins. The proenzyme has an apparent molecular mass of 66 kD, and the mature enzyme has an apparent molecular mass of 46 to 48 kD. Defects in TPP1 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2). This protein distributes widely in all tissues.

实验方案

Product Specific Protocols
WB protocol for TPP1 antibody 12479-1-APDownload protocol
IHC protocol for TPP1 antibody 12479-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Reprod Dev

Protein profiling the differences between diabetic and normal mouse cumulus cells.

Authors - Guang-Jian Jiang
View Article