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验证数据展示

  • WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51644-1

    Cytometric bead array sample test of MP51644-1

    Cytometric bead array sample test of MP51644-1, TPM4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67244-2-PBS. Detection antibody: 67244-1-PBS.

  • WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 67244-1-Ig (same clone as 67244-1-PBS)

    WB analysis of A549 using 67244-1-Ig (same clone as 67244-1-PBS)

    WB result of TPM4 antibody (67244-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM4 transfected A549 cells. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 67244-1-Ig (same clone as 67244-1-PBS)

    IHC staining of mouse heart using 67244-1-Ig (same clone as 67244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67244-1-Ig (TPM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPM4 antibody included
  • IHC staining of rat heart using 67244-1-Ig (same clone as 67244-1-PBS)

    IHC staining of rat heart using 67244-1-Ig (same clone as 67244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67244-1-Ig (TPM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPM4 antibody included
  • IF Staining of MCF-7 using 67244-1-Ig (same clone as 67244-1-PBS)

    IF Staining of MCF-7 using 67244-1-Ig (same clone as 67244-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TPM4 antibody (67244-1-Ig, Clone: 2E12D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51644-1

    Cytometric bead array standard curve of MP51644-1

    Cytometric bead array standard curve of MP51644-1, TPM4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67244-2-PBS. Detection antibody: 67244-1-PBS. Standard:Ag6947. Range: 1.563-200 ng/mL.

  • Featured Product
  • KD/KO验证

TPM4 Monoclonal antibody, PBS Only (Detector)

TPM4 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67244-1-PBS

产品说明书

CloneNo. 2E12D12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

2E12D12, TM30p1, tropomyosin 4, Tropomyosin alpha 4 chain, Tropomyosin alpha-4 chain

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TPM4 Ready-To-Use IHC Kit. TPM4 primary antibody included.

产品信息

67244-1-PBS targets TPM4 as part of a matched antibody pair:

MP51644-1: 67244-2-PBS capture and 67244-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag6947

Product name: Recombinant human TPM4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-248 aa of BC037576

Sequence: MAGLNSLEAVKRKIQALQQQADEAEDRAQGLQRELDGERERREKAEGDVAALNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRAMKDEEKMEIQEMQLKEAKHIAEEADRKYEEVARKLVILEGELERAEERAEVSELKCGDLEEELKNVTNNLKSLEAASEKYSEKEDKYEEEIKLLSDKLKEAETRAEFAERTVAKLEKTIDDLEEKLAQAKEENVGLHQTLDQTLNELNCI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tropomyosin 4
别名 2E12D12, TM30p1, tropomyosin 4, Tropomyosin alpha 4 chain, Tropomyosin alpha-4 chain
计算分子量 248 aa, 29 kDa
观测分子量 32-35 kDa
GenBank蛋白编号BC037576
基因名称 TPM4
Gene ID (NCBI) 7171
ENSEMBL Gene IDENSG00000167460
RRIDAB_2882523
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP67936
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TPM4 is a member of tropomyosins (TPMs), a multi-gene family of actin-binding proteins present in all eukaryotic cells. In muscle, TPMs are responsible for mediating contraction via regulation of the actin-myosin interaction. As to non-muscle cells, its proposed role is to stabilize the actin filaments by modulating the interaction with proteins that are responsible for the regulation of actin dynamics. The form of TPM4 in muscle has a higher molecular weight than the form found in non-muscle cells.

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