验证数据展示
经过测试的应用
| Positive WB detected in | pig heart tissue, U2OS cells, Rabbit heart tissue, Rat heart tissue, Mouse heart tissue |
| Positive IF/ICC detected in | C2C12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68158-1-Ig targets TPM3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 免疫原 |
CatNo: Ag28568 Product name: Recombinant human TPM3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-285 aa of BC008407 Sequence: MMEAIKKKMQMLKLDKENALDRAEQAEAEQKQAEERSKQLEDELAAMQKKLKGTEDELDKYSEALKDAQEKLELAEKKAADAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEDELYAQKLKYKAISEELDHALNDMTSI 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | tropomyosin 3 |
| 别名 | Tropomyosin-5, Tropomyosin-3, Tropomyosin alpha-3 chain, Tropomyosin alpha 3 chain, Gamma-tropomyosin |
| 计算分子量 | 33 kDa |
| 观测分子量 | 33-37 kDa |
| GenBank蛋白编号 | BC008407 |
| 基因名称 | TPM3 |
| Gene ID (NCBI) | 7170 |
| RRID | AB_3085044 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P06753 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TPM3 antibody 68158-1-Ig | Download protocol |
| WB protocol for TPM3 antibody 68158-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
