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TPM1 Polyclonal antibody

TPM1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (2)

Applications

IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 28477-1-AP

Print datasheet

Synonyms

Alpha tropomyosin, C15orf13, CMD1Y, HTM alpha, TMSA, TPM1, Tropomyosin 1, tropomyosin 1 (alpha), Tropomyosin alpha 1 chain

验证数据展示

  • IHC staining of human stomach cancer using 28477-1-AP

    IHC staining of human stomach cancer using 28477-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28477-1-AP (TPM1 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 28477-1-AP

    WB analysis using 28477-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28477-1-AP (TPM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inU-251 cells, mouse heart, mouse lung, rat lung
Positive IHC detected inhuman stomach cancer tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28477-1-AP targets TPM1 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IF, CoIP
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit
Immunogen TPM1 fusion protein Ag29525 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tropomyosin 1 (alpha)
Synonyms Alpha tropomyosin, C15orf13, CMD1Y, HTM alpha, TMSA, TPM1, Tropomyosin 1, tropomyosin 1 (alpha), Tropomyosin alpha 1 chain
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC053545
Gene Symbol TPM1
Gene ID (NCBI) 7168
RRIDAB_2881151
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09493
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TPM1 antibody 28477-1-APDownload protocol
IHC protocol for TPM1 antibody 28477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Bone Res

Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma

Authors - Ming Luo
View Article
WB

Genes Dev

Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein

Authors - Megan L Norris
View Article
ratWB

Genomics

Comprehensive analysis of transcriptome-wide N6-methyladenosine methylomes in the Barrett's esophagus in rats

Authors - Ke Zou
View Article
mouse,rabbitWB,IF,CoIP

Adv Sci (Weinh)

A Circular RNA Generated from Nebulin (NEB) Gene Splicing Promotes Skeletal Muscle Myogenesis in Cattle as Detected by a Multi-Omics Approach

Authors - Kongwei Huang
View Article