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验证数据展示

  • WB analysis using 29283-1-AP

    WB analysis using 29283-1-AP

    Rat brain tissue were subjected to SDS PAGE followed by western blot with 29283-1-AP (TPH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 29283-1-AP

    IHC staining of mouse brain using 29283-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29283-1-AP (TPH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TPH2 Polyclonal antibody

TPH2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29283-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

EC:1.14.16.4, NTPH, TPH 2, Tryptophan 5-hydroxylase 2, Tryptophan 5-monooxygenase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29283-1-AP targets TPH2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TPH2 fusion protein Ag30281 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tryptophan hydroxylase 2
别名 EC:1.14.16.4, NTPH, TPH 2, Tryptophan 5-hydroxylase 2, Tryptophan 5-monooxygenase 2
计算分子量 490 aa, 56 kDa
观测分子量55 kDa
GenBank蛋白编号BC114442
基因名称 TPH2
Gene ID (NCBI) 121278
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWU9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TPH2 protein is a key enzyme in the synthesis of serotonin in the brain. It is mainly expressed in the brain and is responsible for converting tryptophan to 5-hydroxytryptophan, a precursor of serotonin. TPH2 plays a crucial role in regulating mood, anxiety, and stress responses. Variations in the TPH2 gene have been associated with several psychiatric disorders, including depression and bipolar disorder. Additionally, TPH2 expression can be regulated by various factors, such as hormones and environmental stimuli (PMID: 22241550, PMID: 29775696, PMID: 32119710, PMID: 40204433).

实验方案

Product Specific Protocols
WB protocol for TPH2 antibody 29283-1-APDownload protocol
IHC protocol for TPH2 antibody 29283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols