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  • Featured Product
  • KD/KO Validated

TPCN1 Polyclonal antibody

TPCN1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 23758-1-AP

Print datasheet

Synonyms

KIAA1169, TPC1, TPCN1, two pore segment channel 1

验证数据展示

  • WB analysis of mouse heart using 23758-1-AP

    WB analysis of mouse heart using 23758-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 23758-1-AP (TPCN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 23758-1-AP

    IHC staining of human kidney using 23758-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23758-1-AP (TPCN1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 23758-1-AP

    IF Staining of HepG2 using 23758-1-AP

    Immunofluorescent analysis of HepG2 cells using 23758-1-AP (TPCN1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23758-1-AP is TPCN1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen TPCN1 fusion protein Ag20607 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name two pore segment channel 1
Synonyms KIAA1169, TPC1, TPCN1, two pore segment channel 1
Calculated Molecular Weight 816 aa, 94 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberBC136795
Gene Symbol TPCN1
Gene ID (NCBI) 53373
RRIDAB_2879317
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9ULQ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TPCN1 antibody 23758-1-APDownload protocol
IHC protocol for TPCN1 antibody 23758-1-APDownload protocol
IF protocol for TPCN1 antibody 23758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Sci Adv

CLN7 is an organellar chloride channel regulating lysosomal function.

Authors - Yayu Wang
  • KD Validated
View Article