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验证数据展示

  • IHC staining of human testis using 26818-1-AP

    IHC staining of human testis using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP53RK antibody included
  • IHC staining of human testis using 26818-1-AP

    IHC staining of human testis using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP53RK antibody included
  • WB analysis of Jurkat using 26818-1-AP

    WB analysis of Jurkat using 26818-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26818-1-AP (TP53RK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 26818-1-AP

    IP experiment of Jurkat using 26818-1-AP

    IP result of anti-TP53RK (IP:26818-1-AP, 4ug; Detection:26818-1-AP 1:700) with Jurkat cells lysate 3200ug.

  • IHC staining of human placenta using 26818-1-AP

    IHC staining of human placenta using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP53RK antibody included
  • IHC staining of human stomach cancer using 26818-1-AP

    IHC staining of human stomach cancer using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP53RK antibody included
  • IHC staining of mouse heart using 26818-1-AP

    IHC staining of mouse heart using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP53RK antibody included
  • IHC staining of mouse stomach using 26818-1-AP

    IHC staining of mouse stomach using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP53RK antibody included
  • IHC staining of rat heart using 26818-1-AP

    IHC staining of rat heart using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP53RK antibody included
  • IHC staining of rat stomach using 26818-1-AP

    IHC staining of rat stomach using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP53RK antibody included
  • IHC staining of mouse stomach using 26818-1-AP

    IHC staining of mouse stomach using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP53RK antibody included
  • IHC staining of rat stomach using 26818-1-AP

    IHC staining of rat stomach using 26818-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 26818-1-AP (TP53RK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP53RK antibody included
  • Featured Product
  • KD/KO Validated

TP53RK Polyclonal antibody

TP53RK Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 26818-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

Atypical serine/threonine protein kinase TP53RK, BUD32, C20orf64, EC:2.7.11.1, EC:3.6.-.-

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TP53RK Ready-To-Use IHC Kit. TP53RK primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26818-1-AP targets TP53RK in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag25326

Product name: Recombinant human TP53RK protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 154-253 aa of BC009727

Sequence: HDEDLIHGDLTTSNMLLKPPLEQLNIVLIDFGLSFISALPEDKGVDLYVLEKAFLSTHPNTETVFEAFLKSYSTSSKKARPVLKKLDEVRLRGRKRSMVG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TP53 regulating kinase
别名 Atypical serine/threonine protein kinase TP53RK, BUD32, C20orf64, EC:2.7.11.1, EC:3.6.-.-
计算分子量 28 kDa
观测分子量 30 kDa
GenBank蛋白编号BC009727
基因名称 TP53RK
Gene ID (NCBI) 112858
RRIDAB_2880647
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96S44
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TP53RK antibody 26818-1-APDownload protocol
IP protocol for TP53RK antibody 26818-1-APDownload protocol
WB protocol for TP53RK antibody 26818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Biol Med

Systematic screening for potential therapeutic targets in osteosarcoma through a kinome-wide CRISPR-Cas9 library.

Authors - Yuanzhong Wu
  • KO Validated
View Article
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