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TP53I13 Polyclonal antibody

TP53I13 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16596-1-AP

Print datasheet

Synonyms

DSCP1, TP53I13

验证数据展示

  • IHC staining of human heart using 16596-1-AP

    IHC staining of human heart using 16596-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16596-1-AP (TP53I13 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 16596-1-AP

    IHC staining of human heart using 16596-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16596-1-AP (TP53I13 Antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis using 16596-1-AP

    WB analysis using 16596-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16596-1-AP (TP53I13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16596-1-AP targets TP53I13 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen TP53I13 fusion protein Ag9746 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor protein p53 inducible protein 13
Synonyms DSCP1, TP53I13
Calculated Molecular Weight 393 aa, 42 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC001593
Gene Symbol TP53I13
Gene ID (NCBI) 90313
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBR0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TP53I13 is involved in several processes, including negative regulation of cell cycle, response to UV, and response to xenobiotic stimulus.It may act as a tumor suppressorwhich could inhibits tumor cell growth, when overexpressed.

实验方案

Product Specific Protocols
WB protocol for TP53I13 antibody 16596-1-APDownload protocol
IHC protocol for TP53I13 antibody 16596-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols